                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Recombinant Expression of Defensins in Filamentous Fungi

<130>  10789.204-WO

<160>  24    

<170>  PatentIn version 3.3

<210>  1
<211>  288
<212>  DNA
<213>  Pseudoplectania nigrella


<220>
<221>  CDS
<222>  (1)..(285)

<220>
<221>  sig_peptide
<222>  (1)..(69)

<220>
<221>  mat_peptide
<222>  (166)..(285)

<400>  1
atg caa ttt acc acc atc ctc tcc atc ggt atc acc gtc ttc gga ctt       48
Met Gln Phe Thr Thr Ile Leu Ser Ile Gly Ile Thr Val Phe Gly Leu         
-55                 -50                 -45                 -40         

ctc aac acc gga gcc ttt gca gca ccc cag cct gtt ccc gag gct tac       96
Leu Asn Thr Gly Ala Phe Ala Ala Pro Gln Pro Val Pro Glu Ala Tyr         
                -35                 -30                 -25             

gct gtt tct gat ccc gag gct cat cct gac gat ttt gct ggt atg gat      144
Ala Val Ser Asp Pro Glu Ala His Pro Asp Asp Phe Ala Gly Met Asp         
            -20                 -15                 -10                 

gcg aac caa ctt cag aaa cgt gga ttt gga tgc aat ggt cct tgg gat      192
Ala Asn Gln Leu Gln Lys Arg Gly Phe Gly Cys Asn Gly Pro Trp Asp         
        -5              -1  1               5                           

gag gat gat atg cag tgc cac aat cac tgc aag tct att aag ggt tac      240
Glu Asp Asp Met Gln Cys His Asn His Cys Lys Ser Ile Lys Gly Tyr         
10                  15                  20                  25          

aag gga ggt tat tgt gct aag ggg ggc ttt gtt tgc aag tgt tac tag      288
Lys Gly Gly Tyr Cys Ala Lys Gly Gly Phe Val Cys Lys Cys Tyr             
                30                  35                  40              


<210>  2
<211>  95
<212>  PRT
<213>  Pseudoplectania nigrella

<400>  2

Met Gln Phe Thr Thr Ile Leu Ser Ile Gly Ile Thr Val Phe Gly Leu 
-55                 -50                 -45                 -40 


Leu Asn Thr Gly Ala Phe Ala Ala Pro Gln Pro Val Pro Glu Ala Tyr 
                -35                 -30                 -25     


Ala Val Ser Asp Pro Glu Ala His Pro Asp Asp Phe Ala Gly Met Asp 
            -20                 -15                 -10         


Ala Asn Gln Leu Gln Lys Arg Gly Phe Gly Cys Asn Gly Pro Trp Asp 
        -5              -1  1               5                   


Glu Asp Asp Met Gln Cys His Asn His Cys Lys Ser Ile Lys Gly Tyr 
10                  15                  20                  25  


Lys Gly Gly Tyr Cys Ala Lys Gly Gly Phe Val Cys Lys Cys Tyr 
                30                  35                  40  


<210>  3
<211>  273
<212>  DNA
<213>  Eurotium amstelodami


<220>
<221>  CDS
<222>  (1)..(270)

<220>
<221>  sig_peptide
<222>  (1)..(60)

<220>
<221>  mat_peptide
<222>  (145)..(270)

<400>  3
atg cac ttc acc aag gtc tcc acc att ctt ttt acc atc ttc gcc gcc       48
Met His Phe Thr Lys Val Ser Thr Ile Leu Phe Thr Ile Phe Ala Ala         
            -45                 -40                 -35                 

ggc atc atg gct gct ccc acc gaa gga gtc cgt gag gaa gcc gcc cct       96
Gly Ile Met Ala Ala Pro Thr Glu Gly Val Arg Glu Glu Ala Ala Pro         
        -30                 -25                 -20                     

ggc cag gag gtt tac ccc gac gaa cct cct gct tct ctg acc aag cgt      144
Gly Gln Glu Val Tyr Pro Asp Glu Pro Pro Ala Ser Leu Thr Lys Arg         
    -15                 -10                 -5              -1          

ggc ttc gga tgt cct ggt gat gcc tac cag tgc agt gaa cac tgc agg      192
Gly Phe Gly Cys Pro Gly Asp Ala Tyr Gln Cys Ser Glu His Cys Arg         
1               5                   10                  15              

gcc ctg ggc ggt gga cgc act gga gga tac tgt gct gga cct tgg tat      240
Ala Leu Gly Gly Gly Arg Thr Gly Gly Tyr Cys Ala Gly Pro Trp Tyr         
            20                  25                  30                  

ttg ggt cac cct acc tgc acc tgt tct ttc taa                          273
Leu Gly His Pro Thr Cys Thr Cys Ser Phe                                 
        35                  40                                          


<210>  4
<211>  90
<212>  PRT
<213>  Eurotium amstelodami

<400>  4

Met His Phe Thr Lys Val Ser Thr Ile Leu Phe Thr Ile Phe Ala Ala 
            -45                 -40                 -35         


Gly Ile Met Ala Ala Pro Thr Glu Gly Val Arg Glu Glu Ala Ala Pro 
        -30                 -25                 -20             


Gly Gln Glu Val Tyr Pro Asp Glu Pro Pro Ala Ser Leu Thr Lys Arg 
    -15                 -10                 -5              -1  


Gly Phe Gly Cys Pro Gly Asp Ala Tyr Gln Cys Ser Glu His Cys Arg 
1               5                   10                  15      


Ala Leu Gly Gly Gly Arg Thr Gly Gly Tyr Cys Ala Gly Pro Trp Tyr 
            20                  25                  30          


Leu Gly His Pro Thr Cys Thr Cys Ser Phe 
        35                  40          


<210>  5
<211>  273
<212>  DNA
<213>  Picea glauca


<220>
<221>  CDS
<222>  (1)..(270)

<220>
<221>  sig_peptide
<222>  (1)..(60)

<220>
<221>  mat_peptide
<222>  (151)..(270)

<400>  5
atg aag ttc acc atc tcc atc atc gcc gct ctc gct ttc ttc gcc cag       48
Met Lys Phe Thr Ile Ser Ile Ile Ala Ala Leu Ala Phe Phe Ala Gln         
-50                 -45                 -40                 -35         

gga atc gtg gca gct cct gcg cct atc ccc gag gcc gcc gcg gtg gct       96
Gly Ile Val Ala Ala Pro Ala Pro Ile Pro Glu Ala Ala Ala Val Ala         
                -30                 -25                 -20             

gcc cca gag gcc gag cca aag gcg tta gat gag ctt ccg gag ttg caa      144
Ala Pro Glu Ala Glu Pro Lys Ala Leu Asp Glu Leu Pro Glu Leu Gln         
            -15                 -10                 -5                  

aag cgt ggc ttt ggg tgc aac ggt tgg cct ttc gag gac gat gag cag      192
Lys Arg Gly Phe Gly Cys Asn Gly Trp Pro Phe Glu Asp Asp Glu Gln         
    -1  1               5                   10                          

tgc cat aat cac tgc aag acc att cct ggt tat aag ggt ggc tac tgt      240
Cys His Asn His Cys Lys Thr Ile Pro Gly Tyr Lys Gly Gly Tyr Cys         
15                  20                  25                  30          

gcc aac gtt ggc acc act tgc aag tgc tac tag                          273
Ala Asn Val Gly Thr Thr Cys Lys Cys Tyr                                 
                35                  40                                  


<210>  6
<211>  90
<212>  PRT
<213>  Picea glauca

<400>  6

Met Lys Phe Thr Ile Ser Ile Ile Ala Ala Leu Ala Phe Phe Ala Gln 
-50                 -45                 -40                 -35 


Gly Ile Val Ala Ala Pro Ala Pro Ile Pro Glu Ala Ala Ala Val Ala 
                -30                 -25                 -20     


Ala Pro Glu Ala Glu Pro Lys Ala Leu Asp Glu Leu Pro Glu Leu Gln 
            -15                 -10                 -5          


Lys Arg Gly Phe Gly Cys Asn Gly Trp Pro Phe Glu Asp Asp Glu Gln 
    -1  1               5                   10                  


Cys His Asn His Cys Lys Thr Ile Pro Gly Tyr Lys Gly Gly Tyr Cys 
15                  20                  25                  30  


Ala Asn Val Gly Thr Thr Cys Lys Cys Tyr 
                35                  40  


<210>  7
<211>  189
<212>  DNA
<213>  Crassostrea virginica


<220>
<221>  CDS
<222>  (1)..(186)

<220>
<221>  sig_peptide
<222>  (1)..(66)

<220>
<221>  mat_peptide
<222>  (67)..(186)

<400>  7
atg aaa gtg ttt gta ctt ctt aca ata gca gta atg ctt atg gta tct       48
Met Lys Val Phe Val Leu Leu Thr Ile Ala Val Met Leu Met Val Ser         
        -20                 -15                 -10                     

gcc gac gtt gct ttg gcc ggc ttt ggc tgt cct ttg aac cgg tac cag       96
Ala Asp Val Ala Leu Ala Gly Phe Gly Cys Pro Leu Asn Arg Tyr Gln         
    -5              -1  1               5                   10          

tgt cac tca cat tgc caa tcc att ggt cgt aaa ggt gga tac tgt ggt      144
Cys His Ser His Cys Gln Ser Ile Gly Arg Lys Gly Gly Tyr Cys Gly         
                15                  20                  25              

ggg tgg tgg agt ttt aca tgc aca tgc tac cgc act aaa aag tag          189
Gly Trp Trp Ser Phe Thr Cys Thr Cys Tyr Arg Thr Lys Lys                 
            30                  35                  40                  


<210>  8
<211>  62
<212>  PRT
<213>  Crassostrea virginica

<400>  8

Met Lys Val Phe Val Leu Leu Thr Ile Ala Val Met Leu Met Val Ser 
        -20                 -15                 -10             


Ala Asp Val Ala Leu Ala Gly Phe Gly Cys Pro Leu Asn Arg Tyr Gln 
    -5              -1  1               5                   10  


Cys His Ser His Cys Gln Ser Ile Gly Arg Lys Gly Gly Tyr Cys Gly 
                15                  20                  25      


Gly Trp Trp Ser Phe Thr Cys Thr Cys Tyr Arg Thr Lys Lys 
            30                  35                  40  


<210>  9
<211>  189
<212>  DNA
<213>  Mesobuthus gibbosus


<220>
<221>  CDS
<222>  (1)..(186)

<220>
<221>  sig_peptide
<222>  (1)..(72)

<220>
<221>  mat_peptide
<222>  (73)..(186)

<400>  9
atg aaa acc att gta ctt ctt ttc gtg ttg gct tta gta ttc tgc act       48
Met Lys Thr Ile Val Leu Leu Phe Val Leu Ala Leu Val Phe Cys Thr         
                -20                 -15                 -10             

ctt gaa atg gga atg gtg gaa gcc gga ttc ggt tgt cca ttc aat caa       96
Leu Glu Met Gly Met Val Glu Ala Gly Phe Gly Cys Pro Phe Asn Gln         
            -5              -1  1               5                       

gga aga tgt cac aga cat tgt cga agt att cgt cga aga gga gga tat      144
Gly Arg Cys His Arg His Cys Arg Ser Ile Arg Arg Arg Gly Gly Tyr         
    10                  15                  20                          

tgc gat gga ttt ttg aaa caa aga tgt gtt tgt tat cgg aga taa          189
Cys Asp Gly Phe Leu Lys Gln Arg Cys Val Cys Tyr Arg Arg                 
25                  30                  35                              


<210>  10
<211>  62
<212>  PRT
<213>  Mesobuthus gibbosus

<400>  10

Met Lys Thr Ile Val Leu Leu Phe Val Leu Ala Leu Val Phe Cys Thr 
                -20                 -15                 -10     


Leu Glu Met Gly Met Val Glu Ala Gly Phe Gly Cys Pro Phe Asn Gln 
            -5              -1  1               5               


Gly Arg Cys His Arg His Cys Arg Ser Ile Arg Arg Arg Gly Gly Tyr 
    10                  15                  20                  


Cys Asp Gly Phe Leu Lys Gln Arg Cys Val Cys Tyr Arg Arg 
25                  30                  35              


<210>  11
<211>  198
<212>  DNA
<213>  Crassostrea gigas


<220>
<221>  CDS
<222>  (1)..(195)

<220>
<221>  sig_peptide
<222>  (1)..(66)

<220>
<221>  mat_peptide
<222>  (67)..(195)

<400>  11
atg aaa gta ttc gtt ctt tta aca cta gct gtc ctt ctg atg gtt tct       48
Met Lys Val Phe Val Leu Leu Thr Leu Ala Val Leu Leu Met Val Ser         
        -20                 -15                 -10                     

gca gac atg gct ttt gct gga ttt ggg tgt ccg ggt aac cag tta aag       96
Ala Asp Met Ala Phe Ala Gly Phe Gly Cys Pro Gly Asn Gln Leu Lys         
    -5              -1  1               5                   10          

tgc aac aat cac tgc aag tcc att agt tgt aga gcg ggc tac tgt gat      144
Cys Asn Asn His Cys Lys Ser Ile Ser Cys Arg Ala Gly Tyr Cys Asp         
                15                  20                  25              

gca gcc acg ctc tgg tta aga tgt aca tgt acc gat tgt aat gga aag      192
Ala Ala Thr Leu Trp Leu Arg Cys Thr Cys Thr Asp Cys Asn Gly Lys         
            30                  35                  40                  

aag taa                                                              198
Lys                                                                     
                                                                        


<210>  12
<211>  65
<212>  PRT
<213>  Crassostrea gigas

<400>  12

Met Lys Val Phe Val Leu Leu Thr Leu Ala Val Leu Leu Met Val Ser 
        -20                 -15                 -10             


Ala Asp Met Ala Phe Ala Gly Phe Gly Cys Pro Gly Asn Gln Leu Lys 
    -5              -1  1               5                   10  


Cys Asn Asn His Cys Lys Ser Ile Ser Cys Arg Ala Gly Tyr Cys Asp 
                15                  20                  25      


Ala Ala Thr Leu Trp Leu Arg Cys Thr Cys Thr Asp Cys Asn Gly Lys 
            30                  35                  40          


Lys 
    


<210>  13
<211>  195
<212>  DNA
<213>  Crassostrea virginica


<220>
<221>  CDS
<222>  (1)..(192)

<220>
<221>  sig_peptide
<222>  (1)..(66)

<220>
<221>  mat_peptide
<222>  (67)..(192)

<400>  13
atg aaa gtg ttt gtt ctt cta aca ata gct gtc atg ctt ttg gta tct       48
Met Lys Val Phe Val Leu Leu Thr Ile Ala Val Met Leu Leu Val Ser         
        -20                 -15                 -10                     

gcc gat gta gct act gca gat aac gga tgt ccc cgt cgt ccg aga atc       96
Ala Asp Val Ala Thr Ala Asp Asn Gly Cys Pro Arg Arg Pro Arg Ile         
    -5              -1  1               5                   10          

tgt cac aat cgg tgc ata tac aaa ggt cgt aga ggc gga aaa tgt gtc      144
Cys His Asn Arg Cys Ile Tyr Lys Gly Arg Arg Gly Gly Lys Cys Val         
                15                  20                  25              

gga aag tgg aga agc tta tgc gaa tgc atc tac cca tcg aag gcc ggg      192
Gly Lys Trp Arg Ser Leu Cys Glu Cys Ile Tyr Pro Ser Lys Ala Gly         
            30                  35                  40                  

tga                                                                  195


<210>  14
<211>  64
<212>  PRT
<213>  Crassostrea virginica

<400>  14

Met Lys Val Phe Val Leu Leu Thr Ile Ala Val Met Leu Leu Val Ser 
        -20                 -15                 -10             


Ala Asp Val Ala Thr Ala Asp Asn Gly Cys Pro Arg Arg Pro Arg Ile 
    -5              -1  1               5                   10  


Cys His Asn Arg Cys Ile Tyr Lys Gly Arg Arg Gly Gly Lys Cys Val 
                15                  20                  25      


Gly Lys Trp Arg Ser Leu Cys Glu Cys Ile Tyr Pro Ser Lys Ala Gly 
            30                  35                  40          


<210>  15
<211>  207
<212>  DNA
<213>  Aspergillus oryzae


<220>
<221>  CDS
<222>  (1)..(207)

<220>
<221>  sig_peptide
<222>  (1)..(54)

<220>
<221>  mat_peptide
<222>  (79)..(207)

<400>  15
atg aaa ctt ctg acg gtc gcc ttt tcc ctt ctt ctt ctc ggg caa gtc       48
Met Lys Leu Leu Thr Val Ala Phe Ser Leu Leu Leu Leu Gly Gln Val         
    -25                 -20                 -15                         

cat gcc agt cct ttg gta ctc gac aaa agg tct tcc tgc cag ttg ggt       96
His Ala Ser Pro Leu Val Leu Asp Lys Arg Ser Ser Cys Gln Leu Gly         
-10                 -5              -1  1               5               

gac gtc tgg gac ctc aat gct gca gac gcc gcc tgc agc gct tcg tgt      144
Asp Val Trp Asp Leu Asn Ala Ala Asp Ala Ala Cys Ser Ala Ser Cys         
            10                  15                  20                  

gcc att caa cac ggc gac aaa cac ggc gga cac tgc gat aag aac aag      192
Ala Ile Gln His Gly Asp Lys His Gly Gly His Cys Asp Lys Asn Lys         
        25                  30                  35                      

gtc tgc gtc tgc aat                                                  207
Val Cys Val Cys Asn                                                     
    40                                                                  


<210>  16
<211>  69
<212>  PRT
<213>  Aspergillus oryzae

<400>  16

Met Lys Leu Leu Thr Val Ala Phe Ser Leu Leu Leu Leu Gly Gln Val 
    -25                 -20                 -15                 


His Ala Ser Pro Leu Val Leu Asp Lys Arg Ser Ser Cys Gln Leu Gly 
-10                 -5              -1  1               5       


Asp Val Trp Asp Leu Asn Ala Ala Asp Ala Ala Cys Ser Ala Ser Cys 
            10                  15                  20          


Ala Ile Gln His Gly Asp Lys His Gly Gly His Cys Asp Lys Asn Lys 
        25                  30                  35              


Val Cys Val Cys Asn 
    40              


<210>  17
<211>  362
<212>  DNA
<213>  Artificial

<220>
<223>  Pleactasin encoding sequence with intron (see Example 1)

<400>  17
ggatccacca tgcaatttac caccatcctc tccatcggta tcaccgtctt cggacttctc     60

aacaccggag cctttgcagc accccagccg gtacccgagg cttacgctgt ttctgatccc    120

gaggctcatc ctgacgattt tgctggtatg gatgcgaacc aacttcagaa acgtggattt    180

ggatgcaatg gtccttggga tgaggatgat atgcagtgcc acaagtaaga atcacttata    240

actagtagat taagccaaga gtattggaac tgatgataaa tagtcactgc aagtctatta    300

agggttacaa gggaggttat tgtgctaagg ggggctttgt ttgcaagtgt tactagctcg    360

ag                                                                   362


<210>  18
<211>  303
<212>  DNA
<213>  Artificial

<220>
<223>  Intron-less MluN1 containing sequence encoding Plectasin (see 
       Example 3)

<400>  18
ggatccacca tgcaatttac caccatcctc tccatcggta tcaccgtctt cggactggcc     60

aacaccggag cctttgcagc accccagccg gtacccgagg cttacgctgt ttctgatccc    120

gaggctcatc ctgacgattt tgctggtatg gatgcgaacc aacttcagaa acgtggattt    180

ggatgcaatg gtccttggga tgaggatgat atgcagtgcc acaatcactg caagtctatt    240

aagggttaca agggaggtta ttgtgctaag gggggctttg tttgcaagtg ttactagctc    300

gag                                                                  303


<210>  19
<211>  358
<212>  DNA
<213>  Artificial

<220>
<223>  Plectasin encoding sequence with intron of pMT2899 (see Example 
       3)

<400>  19
ggatccacca tgcaatttac caccatcctc tccatcggta tcaccgtctt cggactgggt     60

atgtacacca cccccttgcg tctgatctgt gacatatgta gctgactggt cagccaacac    120

cggagccttt gcagcacccc agccggtacc cgaggcttac gctgtttctg atcccgaggc    180

tcatcctgac gattttgctg gtatggatgc gaaccaactt cagaaacgtg gatttggatg    240

caatggtcct tgggatgagg atgatatgca gtgccacaat cactgcaagt ctattaaggg    300

ttacaaggga ggttattgtg ctaagggggg ctttgtttgc aagtgttact agctcgag      358


<210>  20
<211>  362
<212>  DNA
<213>  Artificial

<220>
<223>  Plectasin encoding sequence of pMT2900 (see Example 3)

<400>  20
ggatccacca tgcaatttac caccatcctc tccatcggta tcaccgtctt cggactgggt     60

aagaatcact tataactagt agattaagcc aagagtattg gaactgatga taaacagcca    120

acaccggagc ctttgcagca ccccagccgg tacccgaggc ttacgctgtt tctgatcccg    180

aggctcatcc tgacgatttt gctggtatgg atgcgaacca acttcagaaa cgtggatttg    240

gatgcaatgg tccttgggat gaggatgata tgcagtgcca caatcactgc aagtctatta    300

agggttacaa gggaggttat tgtgctaagg ggggctttgt ttgcaagtgt tactagctcg    360

ag                                                                   362


<210>  21
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A (see Example 5)

<400>  21
tcttggatcc accatgcact tcaccaaggt ctcc                                 34


<210>  22
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  Primer B (see Example 5)

<400>  22
tcttctcgag ttagaaagaa caggtgcagg tac                                  33


<210>  23
<211>  386
<212>  DNA
<213>  Eurotium amstelodami


<220>
<221>  CDS
<222>  (10)..(195)

<220>
<221>  sig_peptide
<222>  (10)..(69)

<220>
<221>  mat_peptide
<222>  (154)..(377)

<220>
<221>  Intron
<222>  (196)..(240)

<220>
<221>  CDS
<222>  (241)..(305)

<220>
<221>  Intron
<222>  (306)..(358)

<220>
<221>  CDS
<222>  (359)..(377)

<400>  23
ggatccacc atg cac ttc acc aag gtc tcc acc att ctt ttt acc atc ttc     51
          Met His Phe Thr Lys Val Ser Thr Ile Leu Phe Thr Ile Phe       
                      -45                 -40                 -35       

gcc gcc ggc atc atg gct gct ccc acc gaa gga gtc cgt gag gaa gcc       99
Ala Ala Gly Ile Met Ala Ala Pro Thr Glu Gly Val Arg Glu Glu Ala         
                -30                 -25                 -20             

gcc cct ggc cag gag gtt tac ccc gac gaa cct cct gct tct ctg acc      147
Ala Pro Gly Gln Glu Val Tyr Pro Asp Glu Pro Pro Ala Ser Leu Thr         
            -15                 -10                 -5                  

aag cgt ggc ttc gga tgt cct ggt gat gcc tac cag tgc agt gaa cac      195
Lys Arg Gly Phe Gly Cys Pro Gly Asp Ala Tyr Gln Cys Ser Glu His         
    -1  1               5                   10                          

gtatgtcctg ctgctatcta gagtgaatga agctaatgaa tatag tgc agg gcc ctg    252
                                                  Cys Arg Ala Leu       
                                                  15                    

ggc ggt gga cgc act gga gga tac tgt gct gga cct tgg tat ttg ggt      300
Gly Gly Gly Arg Thr Gly Gly Tyr Cys Ala Gly Pro Trp Tyr Leu Gly         
    20                  25                  30                          

cac cc  gtatgttgcc tagatattta attgtttata gtcctttact gatgagctta       355
His Pro                                                                 
35                                                                      

tag t acc tgc acc tgt tct ttc taactcgag                              386
      Thr Cys Thr Cys Ser Phe                                           
                  40                                                    


<210>  24
<211>  90
<212>  PRT
<213>  Eurotium amstelodami

<400>  24

Met His Phe Thr Lys Val Ser Thr Ile Leu Phe Thr Ile Phe Ala Ala 
            -45                 -40                 -35         


Gly Ile Met Ala Ala Pro Thr Glu Gly Val Arg Glu Glu Ala Ala Pro 
        -30                 -25                 -20             


Gly Gln Glu Val Tyr Pro Asp Glu Pro Pro Ala Ser Leu Thr Lys Arg 
    -15                 -10                 -5              -1  


Gly Phe Gly Cys Pro Gly Asp Ala Tyr Gln Cys Ser Glu His Cys Arg 
1               5                   10                  15      


Ala Leu Gly Gly Gly Arg Thr Gly Gly Tyr Cys Ala Gly Pro Trp Tyr 
            20                  25                  30          


Leu Gly His Pro Thr Cys Thr Cys Ser Phe 
        35                  40          


