                         SEQUENCE LISTING



<110>  Chr. Hansen A/S



<120>  Bacteriophage resistant lactic acid bacteria



<130>  P2130PC00



<160>  2     



<170>  PatentIn version 3.1



<210>  1

<211>  2400

<212>  DNA

<213>  Lactococcus lactis



<220>

<221>  CDS

<222>  (1)..(2400)

<223>  



<400>  1
atg tta aaa aaa gaa tgg caa gcc att tta aag cac aaa ttt ttt att       48
Met Leu Lys Lys Glu Trp Gln Ala Ile Leu Lys His Lys Phe Phe Ile         
1               5                   10                  15              

att gtt att atc gct ttg gca ctt gta cca gca att tat aac tat att       96
Ile Val Ile Ile Ala Leu Ala Leu Val Pro Ala Ile Tyr Asn Tyr Ile         
            20                  25                  30                  

ttc tta ggt tct atg tgg gat cct tac ggt aaa ttg aat gac tta cca      144
Phe Leu Gly Ser Met Trp Asp Pro Tyr Gly Lys Leu Asn Asp Leu Pro         
        35                  40                  45                      

gta gcg gtt gta aat tta gac aag acg tct gaa ttg aac gga aaa aaa      192
Val Ala Val Val Asn Leu Asp Lys Thr Ser Glu Leu Asn Gly Lys Lys         
    50                  55                  60                          

ttc aaa ctt ggt gat gat gtc att gct gaa atg aaa aaa tca aag gat      240
Phe Lys Leu Gly Asp Asp Val Ile Ala Glu Met Lys Lys Ser Lys Asp         
65                  70                  75                  80          

tta gat tat cat ttt gtt tcg gag tat aaa gct tct aaa ggg ata aaa      288
Leu Asp Tyr His Phe Val Ser Glu Tyr Lys Ala Ser Lys Gly Ile Lys         
                85                  90                  95              

aaa ggt gat tat tac atg gtt atc act ttt cca gaa aac ttt tca gaa      336
Lys Gly Asp Tyr Tyr Met Val Ile Thr Phe Pro Glu Asn Phe Ser Glu         
            100                 105                 110                 

aat gca aca acc tta atg aat aag aaa cca aaa acg gtt cag tta gat      384
Asn Ala Thr Thr Leu Met Asn Lys Lys Pro Lys Thr Val Gln Leu Asp         
        115                 120                 125                     

tat caa acg aca cgt ggt cat aac tat att tca tca aaa atg agc gaa      432
Tyr Gln Thr Thr Arg Gly His Asn Tyr Ile Ser Ser Lys Met Ser Glu         
    130                 135                 140                         

agt gcg atg aat cag ctg aaa tca gag gtt tct aaa aat atc acg gaa      480
Ser Ala Met Asn Gln Leu Lys Ser Glu Val Ser Lys Asn Ile Thr Glu         
145                 150                 155                 160         

act tac acc aaa gaa att ttc gct aaa ctt ggt gat atg aag tca gga      528
Thr Tyr Thr Lys Glu Ile Phe Ala Lys Leu Gly Asp Met Lys Ser Gly         
                165                 170                 175             

atg aaa gaa gct tct gac ggt tca aat aaa ttg gct gat gga act tcg      576
Met Lys Glu Ala Ser Asp Gly Ser Asn Lys Leu Ala Asp Gly Thr Ser         
            180                 185                 190                 

tca gca tta aat ggt tca aaa gaa tta agt agt aat ttg aat act tta      624
Ser Ala Leu Asn Gly Ser Lys Glu Leu Ser Ser Asn Leu Asn Thr Leu         
        195                 200                 205                     

tct tca tca agt tta act ttt act gac ggc gca aat aat ttg aat tca      672
Ser Ser Ser Ser Leu Thr Phe Thr Asp Gly Ala Asn Asn Leu Asn Ser         
    210                 215                 220                         

ggc tta aat aaa tat gta tca gga gtg aat caa gct gcc aat ggg gga      720
Gly Leu Asn Lys Tyr Val Ser Gly Val Asn Gln Ala Ala Asn Gly Gly         
225                 230                 235                 240         

caa caa ctt tct tct ggt gca gac caa ttt gtc agt gga acg caa caa      768
Gln Gln Leu Ser Ser Gly Ala Asp Gln Phe Val Ser Gly Thr Gln Gln         
                245                 250                 255             

tta gca gca ggt aca caa aca ctg gct gat aaa tca aaa gag ctg tca      816
Leu Ala Ala Gly Thr Gln Thr Leu Ala Asp Lys Ser Lys Glu Leu Ser         
            260                 265                 270                 

gca gga att tca caa att tct caa ggg tca gaa gcg gtg agt caa ttg      864
Ala Gly Ile Ser Gln Ile Ser Gln Gly Ser Glu Ala Val Ser Gln Leu         
        275                 280                 285                     

caa tcg ggt gtg caa caa tta tct gaa ggc tta agt caa atg gct caa      912
Gln Ser Gly Val Gln Gln Leu Ser Glu Gly Leu Ser Gln Met Ala Gln         
    290                 295                 300                         

aaa aca act tta tcc gaa act caa cag caa aat att agt gct gta caa      960
Lys Thr Thr Leu Ser Glu Thr Gln Gln Gln Asn Ile Ser Ala Val Gln         
305                 310                 315                 320         

aat ggt ctt act gaa tta aat cag gag tta caa aaa aat acg att gac     1008
Asn Gly Leu Thr Glu Leu Asn Gln Glu Leu Gln Lys Asn Thr Ile Asp         
                325                 330                 335             

cca aat ttg gct gcc aat atc caa aat aac ttg aaa ggt ctt gga gct     1056
Pro Asn Leu Ala Ala Asn Ile Gln Asn Asn Leu Lys Gly Leu Gly Ala         
            340                 345                 350                 

acg gtc aca aat cta gct aat gat caa gtg gcg gcc tct aag aca gca     1104
Thr Val Thr Asn Leu Ala Asn Asp Gln Val Ala Ala Ser Lys Thr Ala         
        355                 360                 365                     

gtt gaa agt act gag act ttt aaa act tta agc gaa agt cag aaa aca     1152
Val Glu Ser Thr Glu Thr Phe Lys Thr Leu Ser Glu Ser Gln Lys Thr         
    370                 375                 380                         

gat ata aga aat gca tta gat aat tct gtt ggt gct gga gca gtt caa     1200
Asp Ile Arg Asn Ala Leu Asp Asn Ser Val Gly Ala Gly Ala Val Gln         
385                 390                 395                 400         

acc gat tta cta tct tta aat aag aat att aaa gga gtc caa gac agt     1248
Thr Asp Leu Leu Ser Leu Asn Lys Asn Ile Lys Gly Val Gln Asp Ser         
                405                 410                 415             

ctt aca agt cta cta cca atg ttt gga aaa att gct gat ctt aaa aca     1296
Leu Thr Ser Leu Leu Pro Met Phe Gly Lys Ile Ala Asp Leu Lys Thr         
            420                 425                 430                 

tta gtt cca gga gca agt caa ata att gct gac ctt tca ggt ggt ctg     1344
Leu Val Pro Gly Ala Ser Gln Ile Ile Ala Asp Leu Ser Gly Gly Leu         
        435                 440                 445                     

aac gaa gta aac act aat gta aac aca aaa ttt att cct ggt atc aat     1392
Asn Glu Val Asn Thr Asn Val Asn Thr Lys Phe Ile Pro Gly Ile Asn         
    450                 455                 460                         

cag tta aat gct ggt ttg acg aat ttt aat gac cag tta act tct gga     1440
Gln Leu Asn Ala Gly Leu Thr Asn Phe Asn Asp Gln Leu Thr Ser Gly         
465                 470                 475                 480         

agt caa aaa tta act tct ggt ttt gcc caa tat gct gac gga gtt aat     1488
Ser Gln Lys Leu Thr Ser Gly Phe Ala Gln Tyr Ala Asp Gly Val Asn         
                485                 490                 495             

caa gca aac gct ggc gct caa caa tta gca agt aaa tca aaa gat tta     1536
Gln Ala Asn Ala Gly Ala Gln Gln Leu Ala Ser Lys Ser Lys Asp Leu         
            500                 505                 510                 

caa agc ggg aca atg caa tta gtt tct gga ttg tca caa tta caa gcg     1584
Gln Ser Gly Thr Met Gln Leu Val Ser Gly Leu Ser Gln Leu Gln Ala         
        515                 520                 525                     

aat ggt tca act tta act tca ggt tca aat caa ttg gca ggg ggt gct     1632
Asn Gly Ser Thr Leu Thr Ser Gly Ser Asn Gln Leu Ala Gly Gly Ala         
    530                 535                 540                         

caa caa att tcc tct gtt tca caa caa ttg gca aat gga ggt tca agt     1680
Gln Gln Ile Ser Ser Val Ser Gln Gln Leu Ala Asn Gly Gly Ser Ser         
545                 550                 555                 560         

tta aca agt ggt ttg gga acg cta aat aca ggg gct aat aac ttg tca     1728
Leu Thr Ser Gly Leu Gly Thr Leu Asn Thr Gly Ala Asn Asn Leu Ser         
                565                 570                 575             

aca gcg tta acc aaa gca gat gat act ctc tca gca act aat aat tct     1776
Thr Ala Leu Thr Lys Ala Asp Asp Thr Leu Ser Ala Thr Asn Asn Ser         
            580                 585                 590                 

aat gaa aat gct aaa aaa gta gct gca ccg ttg aaa ctt aaa cat act     1824
Asn Glu Asn Ala Lys Lys Val Ala Ala Pro Leu Lys Leu Lys His Thr         
        595                 600                 605                     

gac cat gac aat gta cca gaa aat ggt gca gga atg aca cca tac atg     1872
Asp His Asp Asn Val Pro Glu Asn Gly Ala Gly Met Thr Pro Tyr Met         
    610                 615                 620                         

att aat gtt gct ctc ttt att ggt gcc ctt gcg aca aat gtt gtg att     1920
Ile Asn Val Ala Leu Phe Ile Gly Ala Leu Ala Thr Asn Val Val Ile         
625                 630                 635                 640         

gga att ggt ttc tca ggt gaa aaa tgg aaa tct ggt cgc gaa ttt atg     1968
Gly Ile Gly Phe Ser Gly Glu Lys Trp Lys Ser Gly Arg Glu Phe Met         
                645                 650                 655             

ttg gcg aaa att ggt aca aat ggt ttg gta gca ctt tta caa gga atc     2016
Leu Ala Lys Ile Gly Thr Asn Gly Leu Val Ala Leu Leu Gln Gly Ile         
            660                 665                 670                 

atc gtc tgg gga gca gtt gct ctt ctt gga ttg aga cca aat cat ttg     2064
Ile Val Trp Gly Ala Val Ala Leu Leu Gly Leu Arg Pro Asn His Leu         
        675                 680                 685                     

tgg gaa atg ttg ttg tca gta ctt tta atc agc ttc gct tac atg gca     2112
Trp Glu Met Leu Leu Ser Val Leu Leu Ile Ser Phe Ala Tyr Met Ala         
    690                 695                 700                         

atc aat act ttc ttc tta acg gct cta ggt aag att ggt gaa ttc ctt     2160
Ile Asn Thr Phe Phe Leu Thr Ala Leu Gly Lys Ile Gly Glu Phe Leu         
705                 710                 715                 720         

atg att gtt gtc tta gtt cta caa ctg gcg acg agt gca gga act tac     2208
Met Ile Val Val Leu Val Leu Gln Leu Ala Thr Ser Ala Gly Thr Tyr         
                725                 730                 735             

ccc tta caa ctt gcg cct aaa ata tat caa gtc atc agt cct tgg tta     2256
Pro Leu Gln Leu Ala Pro Lys Ile Tyr Gln Val Ile Ser Pro Trp Leu         
            740                 745                 750                 

cca atg act tat ggt tta aaa atg tta cgc gaa acc att ggt ttg aat     2304
Pro Met Thr Tyr Gly Leu Lys Met Leu Arg Glu Thr Ile Gly Leu Asn         
        755                 760                 765                     

gga gca att tta cca gaa gca atc cta ttt gta gtt att atc gct ctc     2352
Gly Ala Ile Leu Pro Glu Ala Ile Leu Phe Val Val Ile Ile Ala Leu         
    770                 775                 780                         

ttt act ttc atg ttg agt ttc ttc aag aaa ttc tca cgt ttt gca taa     2400
Phe Thr Phe Met Leu Ser Phe Phe Lys Lys Phe Ser Arg Phe Ala             
785                 790                 795                             


<210>  2

<211>  799

<212>  PRT

<213>  Lactococcus lactis



<400>  2

Met Leu Lys Lys Glu Trp Gln Ala Ile Leu Lys His Lys Phe Phe Ile 
1               5                   10                  15      


Ile Val Ile Ile Ala Leu Ala Leu Val Pro Ala Ile Tyr Asn Tyr Ile 
            20                  25                  30          


Phe Leu Gly Ser Met Trp Asp Pro Tyr Gly Lys Leu Asn Asp Leu Pro 
        35                  40                  45              


Val Ala Val Val Asn Leu Asp Lys Thr Ser Glu Leu Asn Gly Lys Lys 
    50                  55                  60                  


Phe Lys Leu Gly Asp Asp Val Ile Ala Glu Met Lys Lys Ser Lys Asp 
65                  70                  75                  80  


Leu Asp Tyr His Phe Val Ser Glu Tyr Lys Ala Ser Lys Gly Ile Lys 
                85                  90                  95      


Lys Gly Asp Tyr Tyr Met Val Ile Thr Phe Pro Glu Asn Phe Ser Glu 
            100                 105                 110         


Asn Ala Thr Thr Leu Met Asn Lys Lys Pro Lys Thr Val Gln Leu Asp 
        115                 120                 125             


Tyr Gln Thr Thr Arg Gly His Asn Tyr Ile Ser Ser Lys Met Ser Glu 
    130                 135                 140                 


Ser Ala Met Asn Gln Leu Lys Ser Glu Val Ser Lys Asn Ile Thr Glu 
145                 150                 155                 160 


Thr Tyr Thr Lys Glu Ile Phe Ala Lys Leu Gly Asp Met Lys Ser Gly 
                165                 170                 175     


Met Lys Glu Ala Ser Asp Gly Ser Asn Lys Leu Ala Asp Gly Thr Ser 
            180                 185                 190         


Ser Ala Leu Asn Gly Ser Lys Glu Leu Ser Ser Asn Leu Asn Thr Leu 
        195                 200                 205             


Ser Ser Ser Ser Leu Thr Phe Thr Asp Gly Ala Asn Asn Leu Asn Ser 
    210                 215                 220                 


Gly Leu Asn Lys Tyr Val Ser Gly Val Asn Gln Ala Ala Asn Gly Gly 
225                 230                 235                 240 


Gln Gln Leu Ser Ser Gly Ala Asp Gln Phe Val Ser Gly Thr Gln Gln 
                245                 250                 255     


Leu Ala Ala Gly Thr Gln Thr Leu Ala Asp Lys Ser Lys Glu Leu Ser 
            260                 265                 270         


Ala Gly Ile Ser Gln Ile Ser Gln Gly Ser Glu Ala Val Ser Gln Leu 
        275                 280                 285             


Gln Ser Gly Val Gln Gln Leu Ser Glu Gly Leu Ser Gln Met Ala Gln 
    290                 295                 300                 


Lys Thr Thr Leu Ser Glu Thr Gln Gln Gln Asn Ile Ser Ala Val Gln 
305                 310                 315                 320 


Asn Gly Leu Thr Glu Leu Asn Gln Glu Leu Gln Lys Asn Thr Ile Asp 
                325                 330                 335     


Pro Asn Leu Ala Ala Asn Ile Gln Asn Asn Leu Lys Gly Leu Gly Ala 
            340                 345                 350         


Thr Val Thr Asn Leu Ala Asn Asp Gln Val Ala Ala Ser Lys Thr Ala 
        355                 360                 365             


Val Glu Ser Thr Glu Thr Phe Lys Thr Leu Ser Glu Ser Gln Lys Thr 
    370                 375                 380                 


Asp Ile Arg Asn Ala Leu Asp Asn Ser Val Gly Ala Gly Ala Val Gln 
385                 390                 395                 400 


Thr Asp Leu Leu Ser Leu Asn Lys Asn Ile Lys Gly Val Gln Asp Ser 
                405                 410                 415     


Leu Thr Ser Leu Leu Pro Met Phe Gly Lys Ile Ala Asp Leu Lys Thr 
            420                 425                 430         


Leu Val Pro Gly Ala Ser Gln Ile Ile Ala Asp Leu Ser Gly Gly Leu 
        435                 440                 445             


Asn Glu Val Asn Thr Asn Val Asn Thr Lys Phe Ile Pro Gly Ile Asn 
    450                 455                 460                 


Gln Leu Asn Ala Gly Leu Thr Asn Phe Asn Asp Gln Leu Thr Ser Gly 
465                 470                 475                 480 


Ser Gln Lys Leu Thr Ser Gly Phe Ala Gln Tyr Ala Asp Gly Val Asn 
                485                 490                 495     


Gln Ala Asn Ala Gly Ala Gln Gln Leu Ala Ser Lys Ser Lys Asp Leu 
            500                 505                 510         


Gln Ser Gly Thr Met Gln Leu Val Ser Gly Leu Ser Gln Leu Gln Ala 
        515                 520                 525             


Asn Gly Ser Thr Leu Thr Ser Gly Ser Asn Gln Leu Ala Gly Gly Ala 
    530                 535                 540                 


Gln Gln Ile Ser Ser Val Ser Gln Gln Leu Ala Asn Gly Gly Ser Ser 
545                 550                 555                 560 


Leu Thr Ser Gly Leu Gly Thr Leu Asn Thr Gly Ala Asn Asn Leu Ser 
                565                 570                 575     


Thr Ala Leu Thr Lys Ala Asp Asp Thr Leu Ser Ala Thr Asn Asn Ser 
            580                 585                 590         


Asn Glu Asn Ala Lys Lys Val Ala Ala Pro Leu Lys Leu Lys His Thr 
        595                 600                 605             


Asp His Asp Asn Val Pro Glu Asn Gly Ala Gly Met Thr Pro Tyr Met 
    610                 615                 620                 


Ile Asn Val Ala Leu Phe Ile Gly Ala Leu Ala Thr Asn Val Val Ile 
625                 630                 635                 640 


Gly Ile Gly Phe Ser Gly Glu Lys Trp Lys Ser Gly Arg Glu Phe Met 
                645                 650                 655     


Leu Ala Lys Ile Gly Thr Asn Gly Leu Val Ala Leu Leu Gln Gly Ile 
            660                 665                 670         


Ile Val Trp Gly Ala Val Ala Leu Leu Gly Leu Arg Pro Asn His Leu 
        675                 680                 685             


Trp Glu Met Leu Leu Ser Val Leu Leu Ile Ser Phe Ala Tyr Met Ala 
    690                 695                 700                 


Ile Asn Thr Phe Phe Leu Thr Ala Leu Gly Lys Ile Gly Glu Phe Leu 
705                 710                 715                 720 


Met Ile Val Val Leu Val Leu Gln Leu Ala Thr Ser Ala Gly Thr Tyr 
                725                 730                 735     


Pro Leu Gln Leu Ala Pro Lys Ile Tyr Gln Val Ile Ser Pro Trp Leu 
            740                 745                 750         


Pro Met Thr Tyr Gly Leu Lys Met Leu Arg Glu Thr Ile Gly Leu Asn 
        755                 760                 765             


Gly Ala Ile Leu Pro Glu Ala Ile Leu Phe Val Val Ile Ile Ala Leu 
    770                 775                 780                 


Phe Thr Phe Met Leu Ser Phe Phe Lys Lys Phe Ser Arg Phe Ala 
785                 790                 795                 


