                         SEQUENCE LISTING



<110>  Pharmexa A/S

       KLYSNER, Steen 

       VON HOEGEN, Paul  

       VOLDBORG, Bjrn 

       GAUTAM, Anand 


<120>  METHOD FOR DOWN-REGULATING IGE


<130>  P1010PC00


<160>  38    


<170>  PatentIn version 3.1


<210>  1

<211>  428

<212>  PRT

<213>  homo sapiens


<220>

<221>  DOMAIN

<222>  (11)..(116)

<223>  Human IgE heavy chain C1 domain


<220>

<221>  MISC_FEATURE

<222>  (209)..(216)

<223>  Linker between domains C2 and C3


<220>

<221>  MISC_FEATURE

<222>  (205)..(219)

<223>  Epitope including C2C3 linker


<220>

<221>  MISC_FEATURE

<222>  (315)..(323)

<223>  Epitope including C3C4 linker


<220>

<221>  MISC_FEATURE

<222>  (244)..(251)

<223>  Epitope in BC loop


<220>

<221>  MISC_FEATURE

<222>  (272)..(280)

<223>  Epitope in DE loop


<220>

<221>  MISC_FEATURE

<222>  (301)..(311)

<223>  Epitope in FG loop


<220>

<221>  MISC_FEATURE

<222>  (317)..(320)

<223>  Linker between domains C3 and C4


<220>

<221>  DOMAIN

<222>  (321)..(422)

<223>  Human IgE heavy chain C4 domain


<220>

<221>  DOMAIN

<222>  (217)..(316)

<223>  Human IgE heavy chain C3 domain


<220>

<221>  DOMAIN

<222>  (113)..(208)

<223>  Human IgE heavy chain C2 domain


<400>  1

Ala Ser Thr Gln Ser Pro Ser Val Phe Pro Leu Thr Arg Cys Cys Lys 
1               5                   10                  15      

Asn Ile Pro Ser Asn Ala Thr Ser Val Thr Leu Gly Cys Leu Ala Thr 
            20                  25                  30          

Gly Tyr Phe Pro Glu Pro Val Met Val Thr Trp Asp Thr Gly Ser Leu 
        35                  40                  45              

Asn Gly Thr Thr Met Thr Leu Pro Ala Thr Thr Leu Thr Leu Ser Gly 
    50                  55                  60                  

His Tyr Ala Thr Ile Ser Leu Leu Thr Val Ser Gly Ala Trp Ala Lys 
65                  70                  75                  80  

Gln Met Phe Thr Cys Arg Val Ala His Thr Pro Ser Ser Thr Asp Trp 
                85                  90                  95      

Val Asp Asn Lys Thr Phe Ser Val Cys Ser Arg Asp Phe Thr Pro Pro 
            100                 105                 110         

Thr Val Lys Ile Leu Gln Ser Ser Cys Asp Gly Gly Gly His Phe Pro 
        115                 120                 125             

Pro Thr Ile Gln Leu Leu Cys Leu Val Ser Gly Tyr Thr Pro Gly Thr 
    130                 135                 140                 

Ile Asn Ile Thr Trp Leu Glu Asp Gly Gln Val Met Asp Val Asp Leu 
145                 150                 155                 160 

Ser Thr Ala Ser Thr Thr Gln Glu Gly Glu Leu Ala Ser Thr Gln Ser 
                165                 170                 175     

Glu Leu Thr Leu Ser Gln Lys His Trp Leu Ser Asp Arg Thr Tyr Thr 
            180                 185                 190         

Cys Gln Val Thr Tyr Gln Gly His Thr Phe Glu Asp Ser Thr Lys Lys 
        195                 200                 205             

Cys Ala Asp Ser Asn Pro Arg Gly Val Ser Ala Tyr Leu Ser Arg Pro 
    210                 215                 220                 

Ser Pro Phe Asp Leu Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys Leu 
225                 230                 235                 240 

Val Val Asp Leu Ala Pro Ser Lys Gly Thr Val Asn Leu Thr Trp Ser 
                245                 250                 255     

Arg Ala Ser Gly Lys Pro Val Asn His Ser Thr Arg Lys Glu Glu Lys 
            260                 265                 270         

Gln Arg Asn Gly Thr Leu Thr Val Thr Ser Thr Leu Pro Val Gly Thr 
        275                 280                 285             

Arg Asp Trp Ile Glu Gly Glu Thr Tyr Gln Cys Arg Val Thr His Pro 
    290                 295                 300                 

His Leu Pro Arg Ala Leu Met Arg Ser Thr Thr Lys Thr Ser Gly Pro 
305                 310                 315                 320 

Arg Ala Ala Pro Glu Val Tyr Ala Phe Ala Thr Pro Glu Trp Pro Gly 
                325                 330                 335     

Ser Arg Asp Lys Arg Thr Leu Ala Cys Leu Ile Gln Asn Phe Met Pro 
            340                 345                 350         

Glu Asp Ile Ser Val Gln Trp Leu His Asn Glu Val Gln Leu Pro Asp 
        355                 360                 365             

Ala Arg His Ser Thr Thr Gln Pro Arg Lys Thr Lys Gly Ser Gly Phe 
    370                 375                 380                 

Phe Val Phe Ser Arg Leu Glu Val Thr Arg Ala Glu Trp Glu Gln Lys 
385                 390                 395                 400 

Asp Glu Phe Ile Cys Arg Ala Val His Glu Ala Ala Ser Pro Ser Gln 
                405                 410                 415     

Thr Val Gln Arg Ala Val Ser Val Asn Pro Gly Lys 
            420                 425             


<210>  2

<211>  323

<212>  PRT

<213>  homo sapiens


<220>

<221>  DOMAIN

<222>  (8)..(103)

<223>  Human IgE heavy chain C2 domain

<220>

<221>  DOMAIN

<222>  (112)..(211)

<223>  Human IgE heavy chain C3 domain


<220>

<221>  DOMAIN

<222>  (216)..(317)

<223>  Human IgE heavy chain C4 domain


<220>

<221>  MISC_FEATURE

<222>  (104)..(111)

<223>  Linker between domains C2 and C3


<220>

<221>  MISC_FEATURE

<222>  (212)..(215)

<223>  Linker between domains C3 and C4


<220>

<221>  MISC_FEATURE

<222>  (100)..(114)

<223>  Epitope including C2C3 linker


<220>

<221>  MISC_FEATURE

<222>  (210)..(218)

<223>  Epitope including C3C4 linker


<220>

<221>  MISC_FEATURE

<222>  (139)..(145)

<223>  Epitope in BC loop


<220>

<221>  MISC_FEATURE

<222>  (167)..(175)

<223>  Epitope in DE loop


<220>

<221>  MISC_FEATURE

<222>  (196)..(206)

<223>  Epitope in FG loop


<400>  2

Met Arg Asp Phe Thr Pro Pro Thr Val Lys Ile Leu Gln Ser Ser Cys 
1               5                   10                  15      

Asp Gly Gly Gly His Phe Pro Pro Thr Ile Gln Leu Leu Cys Leu Val 
            20                  25                  30          

Ser Gly Tyr Thr Pro Gly Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly 
        35                  40                  45              

Gln Val Met Asp Val Asp Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly 
    50                  55                  60                  

Glu Leu Ala Ser Thr Gln Ser Glu Leu Thr Leu Ser Gln Lys His Trp 
65                  70                  75                  80  

Leu Ser Asp Arg Thr Tyr Thr Cys Gln Val Thr Tyr Gln Gly His Thr 
                85                  90                  95      

Phe Glu Asp Ser Thr Lys Lys Cys Ala Asp Ser Asn Pro Arg Gly Val 
            100                 105                 110         

Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys 
        115                 120                 125             

Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala Pro Ser Lys Gly 
    130                 135                 140                 

Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys Pro Val Asn His 
145                 150                 155                 160 

Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr Leu Thr Val Thr 
                165                 170                 175     

Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr 
            180                 185                 190         

Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala Leu Met Arg Ser 
        195                 200                 205             

Thr Thr Lys Thr Ser Gly Pro Arg Ala Ala Pro Glu Val Tyr Ala Phe 
    210                 215                 220                 

Ala Thr Pro Glu Trp Pro Gly Ser Arg Asp Lys Arg Thr Leu Ala Cys 
225                 230                 235                 240 

Leu Ile Gln Asn Phe Met Pro Glu Asp Ile Ser Val Gln Trp Leu His 
                245                 250                 255     

Asn Glu Val Gln Leu Pro Asp Ala Arg His Ser Thr Thr Gln Pro Arg 
            260                 265                 270         

Lys Thr Lys Gly Ser Gly Phe Phe Val Phe Ser Arg Leu Glu Val Thr 
        275                 280                 285             

Arg Ala Glu Trp Glu Gln Lys Asp Glu Phe Ile Cys Arg Ala Val His 
    290                 295                 300                 

Glu Ala Ala Ser Pro Ser Gln Thr Val Gln Arg Ala Val Ser Val Asn 
305                 310                 315                 320 

Pro Gly Lys 
            

<210>  3

<211>  975

<212>  DNA

<213>  Artificial


<220>

<221>  CDS

<222>  (1)..(975)

<223>  Artificial DNA sequence codon optimized for expression in 
       mammalian cells of human IgE heavy chain fragment spanning
       C2, C3, and C4.

<400>  3
atg cgg gac ttt act cct cca acc gtg aaa att ctt cag agc agc tgc       48
Met Arg Asp Phe Thr Pro Pro Thr Val Lys Ile Leu Gln Ser Ser Cys         
1               5                   10                  15              

gat gga ggg gga cac ttc ccc cct aca att cag ctc ctg tgt ctg gtc       96
Asp Gly Gly Gly His Phe Pro Pro Thr Ile Gln Leu Leu Cys Leu Val         
            20                  25                  30                  

agt ggt tac aca cca ggc act atc aat atc acc tgg ctg gaa gat ggc      144
Ser Gly Tyr Thr Pro Gly Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly         
        35                  40                  45                      

cag gtg atg gac gta gac ctc tcc acc gcc tct act acg cag gaa ggc      192
Gln Val Met Asp Val Asp Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly         
    50                  55                  60                          

gaa ctc gca agt act cag tca gag ctc acc ctg tcc caa aag cat tgg      240
Glu Leu Ala Ser Thr Gln Ser Glu Leu Thr Leu Ser Gln Lys His Trp         
65                  70                  75                  80          

ttg tca gat cga acc tat aca tgc cag gtt act tat cag ggc cat acc      288
Leu Ser Asp Arg Thr Tyr Thr Cys Gln Val Thr Tyr Gln Gly His Thr         
                85                  90                  95              

ttc gaa gac agc aca aaa aag tgt gct gac tca aat ccc aga ggg gtc      336
Phe Glu Asp Ser Thr Lys Lys Cys Ala Asp Ser Asn Pro Arg Gly Val         
            100                 105                 110                 

agc gcc tac ctg agc aga cct tct ccc ttc gac ctg ttt atc agg aaa      384
Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys         
        115                 120                 125                     

tcc cct acg atc act tgt ctt gtg gtc gat ctt gcc cca tct aag ggc      432
Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala Pro Ser Lys Gly         
    130                 135                 140                         

aca gtc aac ctg acc tgg agt cgg gcc tcc gga aag cca gtt aat cat      480
Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys Pro Val Asn His         
145                 150                 155                 160         

tca acc cgg aag gaa gag aaa cag agg aat ggc acc ctc acc gtt acc      528
Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr Leu Thr Val Thr         
                165                 170                 175             

agc aca ctg cct gtg ggc act aga gac tgg ata gaa gga gag act tac      576
Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr         
            180                 185                 190                 

cag tgt cgc gtc aca cat cca cac ctg ccg cga gca ttg atg aga tcc      624
Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala Leu Met Arg Ser         
        195                 200                 205                     

acc aca aag acg agt ggt ccg cgg gct gct cct gag gtt tat gca ttc      672
Thr Thr Lys Thr Ser Gly Pro Arg Ala Ala Pro Glu Val Tyr Ala Phe         
    210                 215                 220                         

gca acc ccc gag tgg cct ggg tcc cga gat aag aga aca ctc gct tgc      720
Ala Thr Pro Glu Trp Pro Gly Ser Arg Asp Lys Arg Thr Leu Ala Cys         
225                 230                 235                 240         

ttg atc caa aac ttt atg ccg gag gat att tcc gtg cag tgg ctg cac      768
Leu Ile Gln Asn Phe Met Pro Glu Asp Ile Ser Val Gln Trp Leu His         
                245                 250                 255             

aac gag gtg cag ctc cct gat gcc cgc cac tct act acc caa ccc cgc      816
Asn Glu Val Gln Leu Pro Asp Ala Arg His Ser Thr Thr Gln Pro Arg         
            260                 265                 270                 

aaa aca aag ggg agc ggg ttt ttc gta ttc tcc cgg ctt gag gtg aca      864
Lys Thr Lys Gly Ser Gly Phe Phe Val Phe Ser Arg Leu Glu Val Thr         
        275                 280                 285                     

cgc gcg gag tgg gag caa aag gac gaa ttt att tgc agg gcc gtg cac      912
Arg Ala Glu Trp Glu Gln Lys Asp Glu Phe Ile Cys Arg Ala Val His         
    290                 295                 300                         

gaa gct gcg tcc ccc tct cag acg gta cag agg gcc gtg tct gtg aac      960
Glu Ala Ala Ser Pro Ser Gln Thr Val Gln Arg Ala Val Ser Val Asn         
305                 310                 315                 320         

ccc ggc aaa tga taa                                                  975
Pro Gly Lys                                                             
                                                                        

<210>  4

<211>  323

<212>  PRT

<213>  Homo sapiens


<220>

<221>  DOMAIN

<222>  (1)..(323)

<223>  Human IgE heavy chain fragment spanning domains C2, C3, and C4.


<400>  4

Met Arg Asp Phe Thr Pro Pro Thr Val Lys Ile Leu Gln Ser Ser Cys 
1               5                   10                  15      

Asp Gly Gly Gly His Phe Pro Pro Thr Ile Gln Leu Leu Cys Leu Val 
            20                  25                  30          

Ser Gly Tyr Thr Pro Gly Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly 
        35                  40                  45              

Gln Val Met Asp Val Asp Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly 
    50                  55                  60                  

Glu Leu Ala Ser Thr Gln Ser Glu Leu Thr Leu Ser Gln Lys His Trp 
65                  70                  75                  80  

Leu Ser Asp Arg Thr Tyr Thr Cys Gln Val Thr Tyr Gln Gly His Thr 
                85                  90                  95      

Phe Glu Asp Ser Thr Lys Lys Cys Ala Asp Ser Asn Pro Arg Gly Val 
            100                 105                 110         

Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys 
        115                 120                 125             

Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala Pro Ser Lys Gly 
    130                 135                 140                 

Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys Pro Val Asn His 
145                 150                 155                 160 

Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr Leu Thr Val Thr 
                165                 170                 175     

Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr 
            180                 185                 190         

Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala Leu Met Arg Ser 
        195                 200                 205             

Thr Thr Lys Thr Ser Gly Pro Arg Ala Ala Pro Glu Val Tyr Ala Phe 
    210                 215                 220                 

Ala Thr Pro Glu Trp Pro Gly Ser Arg Asp Lys Arg Thr Leu Ala Cys 
225                 230                 235                 240 

Leu Ile Gln Asn Phe Met Pro Glu Asp Ile Ser Val Gln Trp Leu His 
                245                 250                 255     

Asn Glu Val Gln Leu Pro Asp Ala Arg His Ser Thr Thr Gln Pro Arg 
            260                 265                 270         

Lys Thr Lys Gly Ser Gly Phe Phe Val Phe Ser Arg Leu Glu Val Thr 
        275                 280                 285             

Arg Ala Glu Trp Glu Gln Lys Asp Glu Phe Ile Cys Arg Ala Val His 
    290                 295                 300                 

Glu Ala Ala Ser Pro Ser Gln Thr Val Gln Arg Ala Val Ser Val Asn 
305                 310                 315                 320 

Pro Gly Lys 
            

<210>  5

<211>  972

<212>  DNA

<213>  Artificial


<220>

<221>  CDS

<222>  (1)..(972)

<223>  Artificial DNA sequence codon optimized for expression in E.
       coli of human IgE heavy chain fragment spanning C2, C3, and C4



<400>  5

atg cgt gac ttc acg ccg ccg act gtc aaa atc ctg cag tcc agt tgc       48
Met Arg Asp Phe Thr Pro Pro Thr Val Lys Ile Leu Gln Ser Ser Cys         
1               5                   10                  15              

gac ggt ggc ggt cat ttc ccg ccg acc atc cag ctg ctg tgc ctg gtt       96
Asp Gly Gly Gly His Phe Pro Pro Thr Ile Gln Leu Leu Cys Leu Val         
            20                  25                  30                  

agc ggt tat acc cct ggc acc atc aat atc acc tgg ctg gaa gac ggt      144
Ser Gly Tyr Thr Pro Gly Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly         
        35                  40                  45                      

cag gtt atg gat gtc gac ctg tct acc gcc tct acc acc cag gaa ggt      192
Gln Val Met Asp Val Asp Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly         
    50                  55                  60                          

gaa ctg gct tct acc cag tct gaa ctg acc ctg tct cag aaa cac tgg      240
Glu Leu Ala Ser Thr Gln Ser Glu Leu Thr Leu Ser Gln Lys His Trp         
65                  70                  75                  80          

ctg tct gac cgt acc tac acc tgt cag gtt acc tat cag ggt cac acc      288
Leu Ser Asp Arg Thr Tyr Thr Cys Gln Val Thr Tyr Gln Gly His Thr         
                85                  90                  95              

ttc gaa gat tct acc aag aaa tgc gct gac tcc aat ccg cgt ggc gtt      336
Phe Glu Asp Ser Thr Lys Lys Cys Ala Asp Ser Asn Pro Arg Gly Val         
            100                 105                 110                 

tct gct tac ctg tct cgt ccg tct ccc ttt gat ctg ttc att cgt aaa      384
Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys         
        115                 120                 125                     

agc ccg acc att acc tgc ctg gtt gtt gac ctg gca cca agc aaa ggt      432
Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala Pro Ser Lys Gly         
    130                 135                 140                         

acc gtt aac ctg acc tgg tct cgt gca agc ggt aaa ccg gtt aac cac      480
Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys Pro Val Asn His         
145                 150                 155                 160         

tct acg cgt aaa gaa gag aag caa cgt aac ggc acc ctg acg gtt acc      528
Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr Leu Thr Val Thr         
                165                 170                 175             

tct acc ctg ccg gtt ggt acc cgt gac tgg atc gaa ggt gaa acc tac      576
Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr         
            180                 185                 190                 

cag tgc cgc gtt acc cac ccg cat ctg ccg cgc gct ctg atg cgt tcg      624
Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala Leu Met Arg Ser         
        195                 200                 205                     

acc acc aaa acc tct ggt ccg cgt gct gct ccg gaa gtt tac gct ttc      672
Thr Thr Lys Thr Ser Gly Pro Arg Ala Ala Pro Glu Val Tyr Ala Phe         
    210                 215                 220                         

gct acc ccg gaa tgg ccg ggc tct cgt gac aaa cgt acc ctg gct tgc      720
Ala Thr Pro Glu Trp Pro Gly Ser Arg Asp Lys Arg Thr Leu Ala Cys         
225                 230                 235                 240         

ctg atc cag aac ttc atg ccg gaa gat att tcc gtt cag tgg ctg cac      768
Leu Ile Gln Asn Phe Met Pro Glu Asp Ile Ser Val Gln Trp Leu His         
                245                 250                 255             

aat gaa gtt caa ctg ccg gac gct cgc cat agt aca acc cag ccg cgt      816
Asn Glu Val Gln Leu Pro Asp Ala Arg His Ser Thr Thr Gln Pro Arg         
            260                 265                 270                 

aaa acg aaa ggt tct ggc ttt ttt gta ttc agc cgt ctg gaa gtt acc      864
Lys Thr Lys Gly Ser Gly Phe Phe Val Phe Ser Arg Leu Glu Val Thr         
        275                 280                 285                     

cgt gct gaa tgg gaa cag aaa gac gaa ttt atc tgc cgc gct gtt cac      912
Arg Ala Glu Trp Glu Gln Lys Asp Glu Phe Ile Cys Arg Ala Val His         
    290                 295                 300                         

gaa gcc gct agt ccg tct cag acc gtt cag cgt gct gtt tct gtt aac      960
Glu Ala Ala Ser Pro Ser Gln Thr Val Gln Arg Ala Val Ser Val Asn         
305                 310                 315                 320         

ccg ggt aaa taa                                                      972
Pro Gly Lys                                                             
                                                                        

<210>  6

<211>  323

<212>  PRT

<213>  Homo sapiens


<220>

<221>  DOMAIN

<222>  (1)..(323)

<223>  Human IgE heavy chain fragment spanning domains C2, C3, and C4.


<400>  6

Met Arg Asp Phe Thr Pro Pro Thr Val Lys Ile Leu Gln Ser Ser Cys 
1               5                   10                  15      

Asp Gly Gly Gly His Phe Pro Pro Thr Ile Gln Leu Leu Cys Leu Val 
            20                  25                  30          

Ser Gly Tyr Thr Pro Gly Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly 
        35                  40                  45              

Gln Val Met Asp Val Asp Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly 
    50                  55                  60                  

Glu Leu Ala Ser Thr Gln Ser Glu Leu Thr Leu Ser Gln Lys His Trp 
65                  70                  75                  80  

Leu Ser Asp Arg Thr Tyr Thr Cys Gln Val Thr Tyr Gln Gly His Thr 
                85                  90                  95      

Phe Glu Asp Ser Thr Lys Lys Cys Ala Asp Ser Asn Pro Arg Gly Val 
            100                 105                 110         

Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys 
        115                 120                 125             

Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala Pro Ser Lys Gly 
    130                 135                 140                 

Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys Pro Val Asn His 
145                 150                 155                 160 

Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr Leu Thr Val Thr 
                165                 170                 175     

Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr 
            180                 185                 190         

Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala Leu Met Arg Ser 
        195                 200                 205             

Thr Thr Lys Thr Ser Gly Pro Arg Ala Ala Pro Glu Val Tyr Ala Phe 
    210                 215                 220                 

Ala Thr Pro Glu Trp Pro Gly Ser Arg Asp Lys Arg Thr Leu Ala Cys 
225                 230                 235                 240 

Leu Ile Gln Asn Phe Met Pro Glu Asp Ile Ser Val Gln Trp Leu His 
                245                 250                 255     

Asn Glu Val Gln Leu Pro Asp Ala Arg His Ser Thr Thr Gln Pro Arg 
            260                 265                 270         

Lys Thr Lys Gly Ser Gly Phe Phe Val Phe Ser Arg Leu Glu Val Thr 
        275                 280                 285             

Arg Ala Glu Trp Glu Gln Lys Asp Glu Phe Ile Cys Arg Ala Val His 
    290                 295                 300                 

Glu Ala Ala Ser Pro Ser Gln Thr Val Gln Arg Ala Val Ser Val Asn 
305                 310                 315                 320 

Pro Gly Lys 

   
<210>  7

<211>  441

<212>  PRT

<213>  homo sapiens


<220>

<221>  DOMAIN

<222>  (11)..(106)

<223>  IgE heavy chain C1 domain


<220>

<221>  DOMAIN

<222>  (113)..(208)

<223>  IgE heavy chain C2 domain


<220>

<221>  DOMAIN

<222>  (217)..(317)

<223>  IgE heavy chain C3 domain


<220>

<221>  DOMAIN

<222>  (321)..(422)

<223>  IgE heavy chain C4 domain


<220>

<221>  DOMAIN

<222>  (427)..(441)

<223>  MIGIS fragment


<220>

<221>  MISC_FEATURE

<222>  (209)..(216)

<223>  Linker between domains C2 and C3

<220>

<221>  MISC_FEATURE

<222>  (318)..(320)

<223>  Linker between domains C3 and C4


<220>

<221>  MISC_FEATURE

<222>  (205)..(219)

<223>  Epitope including C2C3 linker



<220>

<221>  MISC_FEATURE

<222>  (315)..(323)

<223>  Epitope including C3C4 linker


<220>

<221>  MISC_FEATURE

<222>  (244)..(251)

<223>  Epitope in BC loop


<220>

<221>  MISC_FEATURE

<222>  (272)..(280)

<223>  Epitope in DE loop


<220>

<221>  MISC_FEATURE

<222>  (301)..(311)

<223>  Epitope in FG loop


<400>  7

Ala Ser Thr Gln Ser Pro Ser Val Phe Pro Leu Thr Arg Cys Cys Lys 
1               5                   10                  15      

Asn Ile Pro Ser Asn Ala Thr Ser Val Thr Leu Gly Cys Leu Ala Thr 
            20                  25                  30          

Gly Tyr Phe Pro Glu Pro Val Met Val Thr Trp Asp Thr Gly Ser Leu 
        35                  40                  45              

Asn Gly Thr Thr Met Thr Leu Pro Ala Thr Thr Leu Thr Leu Ser Gly 
    50                  55                  60                  

His Tyr Ala Thr Ile Ser Leu Leu Thr Val Ser Gly Ala Trp Ala Lys 
65                  70                  75                  80  

Gln Met Phe Thr Cys Arg Val Ala His Thr Pro Ser Ser Thr Asp Trp 
                85                  90                  95      

Val Asp Asn Lys Thr Phe Ser Val Cys Ser Arg Asp Phe Thr Pro Pro 
            100                 105                 110         

Thr Val Lys Ile Leu Gln Ser Ser Cys Asp Gly Gly Gly His Phe Pro 
        115                 120                 125             

Pro Thr Ile Gln Leu Leu Cys Leu Val Ser Gly Tyr Thr Pro Gly Thr 
    130                 135                 140                 

Ile Asn Ile Thr Trp Leu Glu Asp Gly Gln Val Met Asp Val Asp Leu 
145                 150                 155                 160 

Ser Thr Ala Ser Thr Thr Gln Glu Gly Glu Leu Ala Ser Thr Gln Ser 
                165                 170                 175     

Glu Leu Thr Leu Ser Gln Lys His Trp Leu Ser Asp Arg Thr Tyr Thr 
            180                 185                 190         

Cys Gln Val Thr Tyr Gln Gly His Thr Phe Glu Asp Ser Thr Lys Lys 
        195                 200                 205             

Cys Ala Asp Ser Asn Pro Arg Gly Val Ser Ala Tyr Leu Ser Arg Pro 
    210                 215                 220                 

Ser Pro Phe Asp Leu Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys Leu 
225                 230                 235                 240 

Val Val Asp Leu Ala Pro Ser Lys Gly Thr Val Asn Leu Thr Trp Ser 
                245                 250                 255     

Arg Ala Ser Gly Lys Pro Val Asn His Ser Thr Arg Lys Glu Glu Lys 
            260                 265                 270         

Gln Arg Asn Gly Thr Leu Thr Val Thr Ser Thr Leu Pro Val Gly Thr 
        275                 280                 285             

Arg Asp Trp Ile Glu Gly Glu Thr Tyr Gln Cys Arg Val Thr His Pro 
    290                 295                 300                 

His Leu Pro Arg Ala Leu Met Arg Ser Thr Thr Lys Thr Ser Gly Pro 
305                 310                 315                 320 

Arg Ala Ala Pro Glu Val Tyr Ala Phe Ala Thr Pro Glu Trp Pro Gly 
                325                 330                 335     

Ser Arg Asp Lys Arg Thr Leu Ala Cys Leu Ile Gln Asn Phe Met Pro 
            340                 345                 350         

Glu Asp Ile Ser Val Gln Trp Leu His Asn Glu Val Gln Leu Pro Asp 
        355                 360                 365             

Ala Arg His Ser Thr Thr Gln Pro Arg Lys Thr Lys Gly Ser Gly Phe 
    370                 375                 380                 

Phe Val Phe Ser Arg Leu Glu Val Thr Arg Ala Glu Trp Glu Gln Lys 
385                 390                 395                 400 

Asp Glu Phe Ile Cys Arg Ala Val His Glu Ala Ala Ser Pro Ser Gln 
                405                 410                 415     

Thr Val Gln Arg Ala Val Ser Val Asn Pro Glu Leu Asp Val Cys Val 
            420                 425                 430         

Glu Glu Ala Glu Gly Glu Ala Pro Trp 
        435                 440     


<210>  8

<211>  336

<212>  PRT

<213>  homo sapiens


<220>

<221>  DOMAIN

<222>  (8)..(103)

<223>  IgE heavy chain C2 domain


<220>

<221>  DOMAIN

<222>  (112)..(211)

<223>  IgE heavy chain C3 domain


<220>

<221>  DOMAIN

<222>  (216)..(317)

<223>  IgE heavy chain C4 domain


<220>

<221>  DOMAIN

<222>  (322)..(336)

<223>  


<220>

<221>  MISC_FEATURE

<222>  (104)..(111)

<223>  Linker between domains C2 and C3


<220>

<221>  MISC_FEATURE

<222>  (212)..(215)

<223>  Linker between domains C3 and C4


<220>

<221>  MISC_FEATURE

<222>  (100)..(114)

<223>  Epitope including C2C3 linker


<220>

<221>  MISC_FEATURE

<222>  (210)..(218)

<223>  Epitope including C3C4 linker


<220>

<221>  MISC_FEATURE

<222>  (139)..(145)

<223>  Epitope i BC loop


<220>

<221>  MISC_FEATURE

<222>  (167)..(175)

<223>  Epitope i DE loop


<220>

<221>  MISC_FEATURE

<222>  (196)..(206)

<223>  Epitope i FG loop


<400>  8

Met Arg Asp Phe Thr Pro Pro Thr Val Lys Ile Leu Gln Ser Ser Cys 
1               5                   10                  15      

Asp Gly Gly Gly His Phe Pro Pro Thr Ile Gln Leu Leu Cys Leu Val 
            20                  25                  30          

Ser Gly Tyr Thr Pro Gly Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly 
        35                  40                  45              

Gln Val Met Asp Val Asp Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly 
    50                  55                  60                  

Glu Leu Ala Ser Thr Gln Ser Glu Leu Thr Leu Ser Gln Lys His Trp 
65                  70                  75                  80  

Leu Ser Asp Arg Thr Tyr Thr Cys Gln Val Thr Tyr Gln Gly His Thr 
                85                  90                  95      

Phe Glu Asp Ser Thr Lys Lys Cys Ala Asp Ser Asn Pro Arg Gly Val 
            100                 105                 110         

Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys 
        115                 120                 125             

Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala Pro Ser Lys Gly 
    130                 135                 140                 

Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys Pro Val Asn His 
145                 150                 155                 160 

Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr Leu Thr Val Thr 
                165                 170                 175     

Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr 
            180                 185                 190         

Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala Leu Met Arg Ser 
        195                 200                 205             

Thr Thr Lys Thr Ser Gly Pro Arg Ala Ala Pro Glu Val Tyr Ala Phe 
    210                 215                 220                 

Ala Thr Pro Glu Trp Pro Gly Ser Arg Asp Lys Arg Thr Leu Ala Cys 
225                 230                 235                 240 

Leu Ile Gln Asn Phe Met Pro Glu Asp Ile Ser Val Gln Trp Leu His 
                245                 250                 255     

Asn Glu Val Gln Leu Pro Asp Ala Arg His Ser Thr Thr Gln Pro Arg 
            260                 265                 270         

Lys Thr Lys Gly Ser Gly Phe Phe Val Phe Ser Arg Leu Glu Val Thr 
        275                 280                 285             

Arg Ala Glu Trp Glu Gln Lys Asp Glu Phe Ile Cys Arg Ala Val His 
    290                 295                 300                 

Glu Ala Ala Ser Pro Ser Gln Thr Val Gln Arg Ala Val Ser Val Asn 
305                 310                 315                 320 

Pro Glu Leu Asp Val Cys Val Glu Glu Ala Glu Gly Glu Ala Pro Trp 
                325                 330                 335     


<210>  9

<211>  996

<212>  DNA

<213>  Artificial


<220>

<221>  CDS

<222>  (1)..(996)

<223>  Artificial DNA sequence with codons optimised for expression in 
       mammalian cells of human IgE fragment spanning C2, C3, C4 and 
       MIGIS.


<400>  9
atg cgg gac ttt act cct cca acc gtg aaa att ctt cag agc agc tgc       48
Met Arg Asp Phe Thr Pro Pro Thr Val Lys Ile Leu Gln Ser Ser Cys         
1               5                   10                  15              

gat gga ggg gga cac ttc ccc cct aca att cag ctc ctg tgt ctg gtc       96
Asp Gly Gly Gly His Phe Pro Pro Thr Ile Gln Leu Leu Cys Leu Val         
            20                  25                  30                  

agt ggt tac aca cca ggc act atc aat atc acc tgg ctg gaa gat ggc      144
Ser Gly Tyr Thr Pro Gly Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly         
        35                  40                  45                      

cag gtg atg gac gta gac ctc tcc acc gcc tct act acg cag gaa ggc      192
Gln Val Met Asp Val Asp Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly         
    50                  55                  60                          

gaa ctc gca agt act cag tca gag ctc acc ctg tcc caa aag cat tgg      240
Glu Leu Ala Ser Thr Gln Ser Glu Leu Thr Leu Ser Gln Lys His Trp         
65                  70                  75                  80          

ttg tca gat cga acc tat aca tgc cag gtt act tat cag ggc cat acc      288
Leu Ser Asp Arg Thr Tyr Thr Cys Gln Val Thr Tyr Gln Gly His Thr         
                85                  90                  95              

ttc gaa gac agc aca aaa aag tgt gct gac tca aat ccc aga ggg gtc      336
Phe Glu Asp Ser Thr Lys Lys Cys Ala Asp Ser Asn Pro Arg Gly Val         
            100                 105                 110                 

agc gcc tac ctg agc aga cct tct ccc ttc gac ctg ttt atc agg aaa      384
Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys         
        115                 120                 125                     

tcc cct acg atc act tgt ctt gtg gtc gat ctt gcc cca tct aag ggc      432
Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala Pro Ser Lys Gly         
    130                 135                 140                         

aca gtc aac ctg acc tgg agt cgg gcc tcc gga aag cca gtt aat cat      480
Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys Pro Val Asn His         
145                 150                 155                 160         

tca acc cgg aag gaa gag aaa cag agg aat ggc acc ctc acc gtt acc      528
Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr Leu Thr Val Thr         
                165                 170                 175             

agc aca ctg cct gtg ggc act aga gac tgg ata gaa gga gag act tac      576
Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr         
            180                 185                 190                 

cag tgt cgc gtc aca cat cca cac ctg ccg cga gca ttg atg aga tcc      624
Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala Leu Met Arg Ser         
        195                 200                 205                     

acc aca aag acg agt ggt ccg cgg gct gct cct gag gtt tat gca ttc      672
Thr Thr Lys Thr Ser Gly Pro Arg Ala Ala Pro Glu Val Tyr Ala Phe         
    210                 215                 220                         

gca acc ccc gag tgg cct ggg tcc cga gat aag aga aca ctc gct tgc      720
Ala Thr Pro Glu Trp Pro Gly Ser Arg Asp Lys Arg Thr Leu Ala Cys         
225                 230                 235                 240         

ttg atc caa aac ttt atg ccg gag gat att tcc gtg cag tgg ctg cac      768
Leu Ile Gln Asn Phe Met Pro Glu Asp Ile Ser Val Gln Trp Leu His         
                245                 250                 255             

aac gag gtg cag ctc cct gat gcc cgc cac tct act acc caa ccc cgc      816
Asn Glu Val Gln Leu Pro Asp Ala Arg His Ser Thr Thr Gln Pro Arg         
            260                 265                 270                 

aaa aca aag ggg agc ggg ttt ttc gta ttc tcc cgg ctt gag gtg aca      864
Lys Thr Lys Gly Ser Gly Phe Phe Val Phe Ser Arg Leu Glu Val Thr         
        275                 280                 285                     

cgc gcg gag tgg gag caa aag gac gaa ttt att tgc agg gcc gtg cac      912
Arg Ala Glu Trp Glu Gln Lys Asp Glu Phe Ile Cys Arg Ala Val His         
    290                 295                 300                         

gaa gct gcg tcc ccc tct cag acg gta cag agg gag ctg gac gtg tgc      960
Glu Ala Ala Ser Pro Ser Gln Thr Val Gln Arg Glu Leu Asp Val Cys         
305                 310                 315                 320         

gtg gag gag gcc gag ggc gag gcc ccc tgg tga taa                      996
Val Glu Glu Ala Glu Gly Glu Ala Pro Trp                                 
                325                 330                                 

<210>  10

<211>  330

<212>  PRT

<213>  Homo sapiens


<220>

<221>  DOMAIN

<222>  (1)..(330)

<223>  Human IgE heavy chain fragment spanning domains C2, C3, C4, and
       MIGIS.


<400>  10

Met Arg Asp Phe Thr Pro Pro Thr Val Lys Ile Leu Gln Ser Ser Cys 
1               5                   10                  15      

Asp Gly Gly Gly His Phe Pro Pro Thr Ile Gln Leu Leu Cys Leu Val 
            20                  25                  30          

Ser Gly Tyr Thr Pro Gly Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly 
        35                  40                  45              

Gln Val Met Asp Val Asp Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly 
    50                  55                  60                  

Glu Leu Ala Ser Thr Gln Ser Glu Leu Thr Leu Ser Gln Lys His Trp 
65                  70                  75                  80  

Leu Ser Asp Arg Thr Tyr Thr Cys Gln Val Thr Tyr Gln Gly His Thr 
                85                  90                  95      

Phe Glu Asp Ser Thr Lys Lys Cys Ala Asp Ser Asn Pro Arg Gly Val 
            100                 105                 110         

Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys 
        115                 120                 125             

Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala Pro Ser Lys Gly 
    130                 135                 140                 

Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys Pro Val Asn His 
145                 150                 155                 160 

Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr Leu Thr Val Thr 
                165                 170                 175     

Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr 
            180                 185                 190         

Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala Leu Met Arg Ser 
        195                 200                 205             

Thr Thr Lys Thr Ser Gly Pro Arg Ala Ala Pro Glu Val Tyr Ala Phe 
    210                 215                 220                 

Ala Thr Pro Glu Trp Pro Gly Ser Arg Asp Lys Arg Thr Leu Ala Cys 
225                 230                 235                 240 

Leu Ile Gln Asn Phe Met Pro Glu Asp Ile Ser Val Gln Trp Leu His 
                245                 250                 255     

Asn Glu Val Gln Leu Pro Asp Ala Arg His Ser Thr Thr Gln Pro Arg 
            260                 265                 270         

Lys Thr Lys Gly Ser Gly Phe Phe Val Phe Ser Arg Leu Glu Val Thr 
        275                 280                 285             

Arg Ala Glu Trp Glu Gln Lys Asp Glu Phe Ile Cys Arg Ala Val His 
    290                 295                 300                 

Glu Ala Ala Ser Pro Ser Gln Thr Val Gln Arg Glu Leu Asp Val Cys 
305                 310                 315                 320 

Val Glu Glu Ala Glu Gly Glu Ala Pro Trp 
                325                 330 


<210>  11

<211>  171

<212>  DNA

<213>  Artificial


<220>

<221>  CDS

<222>  (1)..(165)

<223>  Synthetic DNA sequence encoding artificial sequence


<220>

<221>  misc_structure

<222>  (4)..(36)

<223>  Epitope in human IgE heavy chain FG loop


<220>

<221>  misc_structure

<222>  (76)..(99)

<223>  Epitope in human IgE heavy chain BC loop


<220>

<221>  misc_structure

<222>  (142)..(165)

<223>  Epitope in human IgE heavy chain DE loop


<220>

<221>  misc_structure

<222>  (37)..(75)

<223>  PADRE epitope


<400>  11

atg gtc aca cat cca cac ctg ccg cga gca ttg atg gct aag ttc gtg       48
Met Val Thr His Pro His Leu Pro Arg Ala Leu Met Ala Lys Phe Val         
1               5                   10                  15              

gcc gct tgg acc ctg aag gcc gca gct ctt gcc cca tct aag ggc aca       96
Ala Ala Trp Thr Leu Lys Ala Ala Ala Leu Ala Pro Ser Lys Gly Thr         
            20                  25                  30                  

gtc gct aag ttc gtg gcc gct tgg acc ctg aag gcc gca gct aaa cag      144
Val Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Lys Gln         
        35                  40                  45                      

agg aat ggc acc ctc acc gtt tgataa                                   171
Arg Asn Gly Thr Leu Thr Val                                             
    50                  55                                              


<210>  12

<211>  55

<212>  PRT

<213>  Artificial


<220>

<221>  MISC_FEATURE

<222>  (1)..(55)

<223>  Artificial sequence


<220>

<221>  MISC_FEATURE

<222>  (2)..(12)

<223>  Epitope in human IgE heavy chain FG loop


<220>

<221>  MISC_FEATURE

<222>  (26)..(33)

<223>  Epitope in human IgE heavy chain BC loop


<220>

<221>  MISC_FEATURE

<222>  (48)..(55)

<223>  Epitope in human IgE heavy chain DE loop


<220>

<221>  MISC_FEATURE

<222>  (13)..(25)

<223>  PADRE epitope


<400>  12

Met Val Thr His Pro His Leu Pro Arg Ala Leu Met Ala Lys Phe Val 
1               5                   10                  15      

Ala Ala Trp Thr Leu Lys Ala Ala Ala Leu Ala Pro Ser Lys Gly Thr 
            20                  25                  30          

Val Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala Lys Gln 
        35                  40                  45              

Arg Asn Gly Thr Leu Thr Val 
    50                  55  


<210>  13

<211>  45

<212>  DNA

<213>  Artificial



<220>

<221>  CDS

<222>  (1)..(42)

<223>  Synthetic DNA sequence encoding linker between human IgE domains 
       C2 and C3



<400>  13
agc aca aaa aag tgt gct gac tca aat ccc aga ggg gtc agc gcc           45
Ser Thr Lys Lys Cys Ala Asp Ser Asn Pro Arg Gly Val Ser                 
1               5                   10                                  


<210>  14

<211>  14

<212>  PRT

<213>  Homo sapiens


<220>

<221>  MISC_FEATURE

<222>  (1)..(14)

<223>  Linker between human IgE domains C2 and C3


<400>  14

Ser Thr Lys Lys Cys Ala Asp Ser Asn Pro Arg Gly Val Ser 
1               5                   10                  


<210>  15

<211>  27

<212>  DNA

<213>  Artificial


<220>

<221>  CDS

<222>  (1)..(27)

<223>  Synthetic DNA sequence encoding linker between human IgE domains 
       C3 and C4


<400>  15

aca aag acg agt ggt ccg cgg gct gct                                   27
Thr Lys Thr Ser Gly Pro Arg Ala Ala                                     
1               5                                                       


<210>  16

<211>  9

<212>  PRT

<213>  Homo sapiens


<220>

<221>  MISC_FEATURE

<222>  (1)..(9)

<223>  Linker between human IgE domains C3 and C4


<400>  16

Thr Lys Thr Ser Gly Pro Arg Ala Ala 
1               5                   


<210>  17

<211>  39

<212>  DNA

<213>  Artificial


<220>

<221>  CDS

<222>  (1)..(39)

<223>  Synthetic DNA sequence encoding pan DR binding epitope


<400>  17

gct aag ttc gtg gcc gct tgg acc ctg aag gcc gca gct                   39
Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala                     
1               5                   10                                  

<210>  18

<211>  13

<212>  PRT

<213>  Artificial


<220>

<221>  MISC_FEATURE

<222>  (1)..(13)

<223>  synthetic pan DR binding epitope


<400>  18

Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala 
1               5                   10              


<210>  19

<211>  432

<212>  PRT

<213>  mus musculus


<220>

<221>  MISC_FEATURE

<222>  (1)..(432)

<223>  Murine IgE heavy chain, domains C1, C2, C3, C4, and MIGIS
       fragment


<400>  19

Ser Ile Arg Asn Pro Gln Leu Tyr Pro Leu Lys Pro Cys Lys Gly Thr 
1               5                   10                  15      

Ala Ser Met Thr Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Asn Pro 
            20                  25                  30          

Val Thr Val Thr Trp Tyr Ser Asp Ser Leu Asn Met Ser Thr Val Asn 
        35                  40                  45              

Phe Pro Ala Leu Gly Ser Glu Leu Lys Val Thr Thr Ser Gln Val Thr 
    50                  55                  60                  

Ser Trp Gly Lys Ser Ala Lys Asn Phe Thr Cys His Val Thr His Pro 
65                  70                  75                  80  

Pro Ser Phe Asn Glu Ser Arg Thr Ile Leu Val Arg Pro Val Asn Ile 
                85                  90                  95      

Thr Glu Pro Thr Leu Glu Leu Leu His Ser Ser Cys Asp Pro Asn Ala 
            100                 105                 110         

Phe His Ser Thr Ile Gln Leu Tyr Cys Phe Ile Tyr Gly His Ile Leu 
        115                 120                 125             

Asn Asp Val Ser Val Ser Trp Leu Met Asp Asp Arg Glu Ile Thr Asp 
    130                 135                 140                 

Thr Leu Ala Gln Thr Val Leu Ile Lys Glu Glu Gly Lys Leu Ala Ser 
145                 150                 155                 160 

Thr Cys Ser Lys Leu Asn Ile Thr Glu Gln Gln Trp Met Ser Glu Ser 
                165                 170                 175     

Thr Phe Thr Cys Lys Val Thr Ser Gln Gly Val Asp Tyr Leu Ala His 
            180                 185                 190         

Thr Arg Arg Cys Pro Asp His Glu Pro Arg Gly Val Ile Thr Tyr Leu 
        195                 200                 205             

Ile Pro Pro Ser Pro Leu Asp Leu Tyr Gln Asn Gly Ala Pro Lys Leu 
    210                 215                 220                 

Thr Cys Leu Val Val Asp Leu Glu Ser Glu Lys Asn Val Asn Val Thr 
225                 230                 235                 240 

Trp Asn Gln Glu Lys Lys Thr Ser Val Ser Ala Ser Gln Trp Tyr Thr 
                245                 250                 255     

Lys His His Asn Asn Ala Thr Thr Ser Ile Thr Ser Ile Leu Pro Val 
            260                 265                 270         

Val Ala Lys Asp Trp Ile Glu Gly Tyr Gly Tyr Gln Cys Ile Val Asp 
        275                 280                 285             

His Pro Asp Phe Pro Lys Pro Ile Val Arg Ser Ile Thr Lys Thr Pro 
    290                 295                 300                 

Gly Gln Arg Ser Ala Pro Glu Val Tyr Val Phe Pro Pro Pro Glu Glu 
305                 310                 315                 320 

Glu Ser Glu Asp Lys Arg Thr Leu Thr Cys Leu Ile Gln Asn Phe Phe 
                325                 330                 335     

Pro Glu Asp Ile Ser Val Gln Trp Leu Gly Asp Gly Lys Leu Ile Ser 
            340                 345                 350         

Asn Ser Gln His Ser Thr Thr Thr Pro Leu Lys Ser Asn Gly Ser Asn 
        355                 360                 365             

Gln Gly Phe Phe Ile Phe Ser Arg Leu Glu Val Ala Lys Thr Leu Trp 
    370                 375                 380                 

Thr Gln Arg Lys Gln Phe Thr Cys Gln Val Ile His Glu Ala Leu Gln 
385                 390                 395                 400 

Lys Pro Arg Lys Leu Glu Lys Thr Ile Ser Thr Ser Leu Glu Leu Asp 
                405                 410                 415     

Leu Gln Asp Leu Cys Ile Glu Glu Val Glu Gly Glu Glu Leu Glu Glu 
            420                 425                 430         


<210>  20

<211>  343

<212>  PRT

<213>  mus musculus


<220>

<221>  MISC_FEATURE

<223>  Murine IgE heavy chain, domains C2, C3, C4, and MIGIS fragment


<400>  20

Met Val Arg Pro Val Asn Ile Thr Glu Pro Thr Leu Glu Leu Leu His 
1               5                   10                  15      

Ser Ser Cys Asp Pro Asn Ala Phe His Ser Thr Ile Gln Leu Tyr Cys 
            20                  25                  30          

Phe Ile Tyr Gly His Ile Leu Asn Asp Val Ser Val Ser Trp Leu Met 
        35                  40                  45              

Asp Asp Arg Glu Ile Thr Asp Thr Leu Ala Gln Thr Val Leu Ile Lys 
    50                  55                  60                  

Glu Glu Gly Lys Leu Ala Ser Thr Cys Ser Lys Leu Asn Ile Thr Glu 
65                  70                  75                  80  

Gln Gln Trp Met Ser Glu Ser Thr Phe Thr Cys Lys Val Thr Ser Gln 
                85                  90                  95      

Gly Val Asp Tyr Leu Ala His Thr Arg Arg Cys Pro Asp His Glu Pro 
            100                 105                 110         

Arg Gly Val Ile Thr Tyr Leu Ile Pro Pro Ser Pro Leu Asp Leu Tyr 
        115                 120                 125             

Gln Asn Gly Ala Pro Lys Leu Thr Cys Leu Val Val Asp Leu Glu Ser 
    130                 135                 140                 

Glu Lys Asn Val Asn Val Thr Trp Asn Gln Glu Lys Lys Thr Ser Val 
145                 150                 155                 160 

Ser Ala Ser Gln Trp Tyr Thr Lys His His Asn Asn Ala Thr Thr Ser 
                165                 170                 175     

Ile Thr Ser Ile Leu Pro Val Val Ala Lys Asp Trp Ile Glu Gly Tyr 
            180                 185                 190         

Gly Tyr Gln Cys Ile Val Asp His Pro Asp Phe Pro Lys Pro Ile Val 
        195                 200                 205             

Arg Ser Ile Thr Lys Thr Pro Gly Gln Arg Ser Ala Pro Glu Val Tyr 
    210                 215                 220                 

Val Phe Pro Pro Pro Glu Glu Glu Ser Glu Asp Lys Arg Thr Leu Thr 
225                 230                 235                 240 

Cys Leu Ile Gln Asn Phe Phe Pro Glu Asp Ile Ser Val Gln Trp Leu 
                245                 250                 255     

Gly Asp Gly Lys Leu Ile Ser Asn Ser Gln His Ser Thr Thr Thr Pro 
            260                 265                 270         

Leu Lys Ser Asn Gly Ser Asn Gln Gly Phe Phe Ile Phe Ser Arg Leu 
        275                 280                 285             

Glu Val Ala Lys Thr Leu Trp Thr Gln Arg Lys Gln Phe Thr Cys Gln 
    290                 295                 300                 

Val Ile His Glu Ala Leu Gln Lys Pro Arg Lys Leu Glu Lys Thr Ile 
305                 310                 315                 320 

Ser Thr Ser Leu Glu Leu Asp Leu Gln Asp Leu Cys Ile Glu Glu Val 
                325                 330                 335     

Glu Gly Glu Glu Leu Glu Glu 
            340             


<210>  21

<211>  1035

<212>  DNA

<213>  Artificial



<220>

<221>  CDS

<222>  (1)..(1035)

<223>  Artificial DNA sequence codon optimised for mammalian expression 
       of murine IgE heavy chain fragment including domains C2, C3, C4, 
       and MIGIS.


<400>  21

atg gtg aga ccc gtg aac att acc gaa cct aca ctg gag ctg ctc cat       48
Met Val Arg Pro Val Asn Ile Thr Glu Pro Thr Leu Glu Leu Leu His         
1               5                   10                  15              

tcc tct tgt gat cct aac gct ttc cat agc acc att cag ctc tac tgt       96
Ser Ser Cys Asp Pro Asn Ala Phe His Ser Thr Ile Gln Leu Tyr Cys         
            20                  25                  30                  

ttt atc tat ggc cac atc ctg aac gat gtg tct gtc agc tgg ctg atg      144
Phe Ile Tyr Gly His Ile Leu Asn Asp Val Ser Val Ser Trp Leu Met         
        35                  40                  45                      

gat gac cgc gag atc acc gat acc ctc gct cag act gtc ctg atc aaa      192
Asp Asp Arg Glu Ile Thr Asp Thr Leu Ala Gln Thr Val Leu Ile Lys         
    50                  55                  60                          

gaa gag ggc aaa ctc gcc tct act tgt tcc aaa ctg aac atc acc gag      240
Glu Glu Gly Lys Leu Ala Ser Thr Cys Ser Lys Leu Asn Ile Thr Glu         
65                  70                  75                  80          

cag cag tgg atg tcc gaa agc aca ttc acg tgc aag gtg acg agc cag      288
Gln Gln Trp Met Ser Glu Ser Thr Phe Thr Cys Lys Val Thr Ser Gln         
                85                  90                  95              

ggc gtg gac tat ctg gcc cac acc agg cgg tgc ccc gac cac gaa ccc      336
Gly Val Asp Tyr Leu Ala His Thr Arg Arg Cys Pro Asp His Glu Pro         
            100                 105                 110                 

cga ggc gtg att act tac ctg atc cct ccc tcc cct ctg gac ctg tac      384
Arg Gly Val Ile Thr Tyr Leu Ile Pro Pro Ser Pro Leu Asp Leu Tyr         
        115                 120                 125                     

cag aac ggc gct cct aag ctg act tgc ctg gtg gtg gac ctg gag tct      432
Gln Asn Gly Ala Pro Lys Leu Thr Cys Leu Val Val Asp Leu Glu Ser         
    130                 135                 140                         

gag aag aat gtc aat gtc aca tgg aat cag gag aag aag acc tcc gtg      480
Glu Lys Asn Val Asn Val Thr Trp Asn Gln Glu Lys Lys Thr Ser Val         
145                 150                 155                 160         

tct gcc tct cag tgg tac aca aag cac cac aat aac gct acc acc tcc      528
Ser Ala Ser Gln Trp Tyr Thr Lys His His Asn Asn Ala Thr Thr Ser         
                165                 170                 175             

atc aca tct att ctg cca gtg gtc gct aag gac tgg atc gag ggc tat      576
Ile Thr Ser Ile Leu Pro Val Val Ala Lys Asp Trp Ile Glu Gly Tyr         
            180                 185                 190                 

ggc tat cag tgc atc gtc gac cac cca gac ttc ccc aag cct att gtc      624
Gly Tyr Gln Cys Ile Val Asp His Pro Asp Phe Pro Lys Pro Ile Val         
        195                 200                 205                     

aga tct atc aca aag acc cct ggc cag aga agc gct ccc gag gtg tac      672
Arg Ser Ile Thr Lys Thr Pro Gly Gln Arg Ser Ala Pro Glu Val Tyr         
    210                 215                 220                         

gtg ttc ccc cct cca gag gag gag agc gag gat aag aga acc ctg aca      720
Val Phe Pro Pro Pro Glu Glu Glu Ser Glu Asp Lys Arg Thr Leu Thr         
225                 230                 235                 240         

tgt ctg atc cag aat ttt ttt ccc gaa gat att tcc gtg cag tgg ctg      768
Cys Leu Ile Gln Asn Phe Phe Pro Glu Asp Ile Ser Val Gln Trp Leu         
                245                 250                 255             

ggc gat ggc aag ctg att agc aat agc cag cat agc aca aca aca cca      816
Gly Asp Gly Lys Leu Ile Ser Asn Ser Gln His Ser Thr Thr Thr Pro         
            260                 265                 270                 

ctg aaa tcc aac ggc tct aac cag ggc ttt ttc att ttc agc aga ctg      864
Leu Lys Ser Asn Gly Ser Asn Gln Gly Phe Phe Ile Phe Ser Arg Leu         
        275                 280                 285                     

gaa gtg gcc aaa acc ctg tgg acc cag aga aaa cag ttc aca tgc cag      912
Glu Val Ala Lys Thr Leu Trp Thr Gln Arg Lys Gln Phe Thr Cys Gln         
    290                 295                 300                         

gtg atc cat gag gcc ctc cag aaa cca aga aag ctg gaa aag aca atc      960
Val Ile His Glu Ala Leu Gln Lys Pro Arg Lys Leu Glu Lys Thr Ile         
305                 310                 315                 320         

tcc acc tcc ctg gag ctg gac ctc cag gac ctg tgc atc gaa gag gtg     1008
Ser Thr Ser Leu Glu Leu Asp Leu Gln Asp Leu Cys Ile Glu Glu Val         
                325                 330                 335             

gaa ggc gag gaa ctg gaa gag taa tga                                 1035
Glu Gly Glu Glu Leu Glu Glu                                             
            340                                                         


<210>  22

<211>  343

<212>  PRT

<213>  mus musculus


<220>

<221>  DOMAIN

<222>  (1)..(343)

<223>  Murine IgE heavy chain fragment including domains C2, C3, C4, 
       and MIGIS.


<400>  22

Met Val Arg Pro Val Asn Ile Thr Glu Pro Thr Leu Glu Leu Leu His 
1               5                   10                  15      

Ser Ser Cys Asp Pro Asn Ala Phe His Ser Thr Ile Gln Leu Tyr Cys 
            20                  25                  30          

Phe Ile Tyr Gly His Ile Leu Asn Asp Val Ser Val Ser Trp Leu Met 
        35                  40                  45              

Asp Asp Arg Glu Ile Thr Asp Thr Leu Ala Gln Thr Val Leu Ile Lys 
    50                  55                  60                  

Glu Glu Gly Lys Leu Ala Ser Thr Cys Ser Lys Leu Asn Ile Thr Glu 
65                  70                  75                  80  

Gln Gln Trp Met Ser Glu Ser Thr Phe Thr Cys Lys Val Thr Ser Gln 
                85                  90                  95      

Gly Val Asp Tyr Leu Ala His Thr Arg Arg Cys Pro Asp His Glu Pro 
            100                 105                 110         

Arg Gly Val Ile Thr Tyr Leu Ile Pro Pro Ser Pro Leu Asp Leu Tyr 
        115                 120                 125             

Gln Asn Gly Ala Pro Lys Leu Thr Cys Leu Val Val Asp Leu Glu Ser 
    130                 135                 140                 

Glu Lys Asn Val Asn Val Thr Trp Asn Gln Glu Lys Lys Thr Ser Val 
145                 150                 155                 160 

Ser Ala Ser Gln Trp Tyr Thr Lys His His Asn Asn Ala Thr Thr Ser 
                165                 170                 175     

Ile Thr Ser Ile Leu Pro Val Val Ala Lys Asp Trp Ile Glu Gly Tyr 
            180                 185                 190         

Gly Tyr Gln Cys Ile Val Asp His Pro Asp Phe Pro Lys Pro Ile Val 
        195                 200                 205             

Arg Ser Ile Thr Lys Thr Pro Gly Gln Arg Ser Ala Pro Glu Val Tyr 
    210                 215                 220                 

Val Phe Pro Pro Pro Glu Glu Glu Ser Glu Asp Lys Arg Thr Leu Thr 
225                 230                 235                 240 

Cys Leu Ile Gln Asn Phe Phe Pro Glu Asp Ile Ser Val Gln Trp Leu 
                245                 250                 255     

Gly Asp Gly Lys Leu Ile Ser Asn Ser Gln His Ser Thr Thr Thr Pro 
            260                 265                 270         

Leu Lys Ser Asn Gly Ser Asn Gln Gly Phe Phe Ile Phe Ser Arg Leu 
        275                 280                 285             

Glu Val Ala Lys Thr Leu Trp Thr Gln Arg Lys Gln Phe Thr Cys Gln 
    290                 295                 300                 

Val Ile His Glu Ala Leu Gln Lys Pro Arg Lys Leu Glu Lys Thr Ile 
305                 310                 315                 320 

Ser Thr Ser Leu Glu Leu Asp Leu Gln Asp Leu Cys Ile Glu Glu Val 
                325                 330                 335     

Glu Gly Glu Glu Leu Glu Glu 
            340             


<210>  23

<211>  332

<212>  PRT

<213>  mus musculus


<220>

<221>  MISC_FEATURE

<222>  (1)..(332)

<223>  Murine IgE heavy chain domains C2, C3, and C4.


<400>  23

Met Val Arg Pro Val Asn Ile Thr Glu Pro Thr Leu Glu Leu Leu His 
1               5                   10                  15      

Ser Ser Cys Asp Pro Asn Ala Phe His Ser Thr Ile Gln Leu Tyr Cys 
            20                  25                  30          

Phe Ile Tyr Gly His Ile Leu Asn Asp Val Ser Val Ser Trp Leu Met 
        35                  40                  45              

Asp Asp Arg Glu Ile Thr Asp Thr Leu Ala Gln Thr Val Leu Ile Lys 
    50                  55                  60                  

Glu Glu Gly Lys Leu Ala Ser Thr Cys Ser Lys Leu Asn Ile Thr Glu 
65                  70                  75                  80  

Gln Gln Trp Met Ser Glu Ser Thr Phe Thr Cys Lys Val Thr Ser Gln 
                85                  90                  95      

Gly Val Asp Tyr Leu Ala His Thr Arg Arg Cys Pro Asp His Glu Pro 
            100                 105                 110         

Arg Gly Val Ile Thr Tyr Leu Ile Pro Pro Ser Pro Leu Asp Leu Tyr 
        115                 120                 125             

Gln Asn Gly Ala Pro Lys Leu Thr Cys Leu Val Val Asp Leu Glu Ser 
    130                 135                 140                 

Glu Lys Asn Val Asn Val Thr Trp Asn Gln Glu Lys Lys Thr Ser Val 
145                 150                 155                 160 

Ser Ala Ser Gln Trp Tyr Thr Lys His His Asn Asn Ala Thr Thr Ser 
                165                 170                 175     

Ile Thr Ser Ile Leu Pro Val Val Ala Lys Asp Trp Ile Glu Gly Tyr 
            180                 185                 190         

Gly Tyr Gln Cys Ile Val Asp His Pro Asp Phe Pro Lys Pro Ile Val 
        195                 200                 205             

Arg Ser Ile Thr Lys Thr Pro Gly Gln Arg Ser Ala Pro Glu Val Tyr 
    210                 215                 220                 

Val Phe Pro Pro Pro Glu Glu Glu Ser Glu Asp Lys Arg Thr Leu Thr 
225                 230                 235                 240 

Cys Leu Ile Gln Asn Phe Phe Pro Glu Asp Ile Ser Val Gln Trp Leu 
                245                 250                 255     

Gly Asp Gly Lys Leu Ile Ser Asn Ser Gln His Ser Thr Thr Thr Pro 
            260                 265                 270         

Leu Lys Ser Asn Gly Ser Asn Gln Gly Phe Phe Ile Phe Ser Arg Leu 
        275                 280                 285             

Glu Val Ala Lys Thr Leu Trp Thr Gln Arg Lys Gln Phe Thr Cys Gln 
    290                 295                 300                 

Val Ile His Glu Ala Leu Gln Lys Pro Arg Lys Leu Glu Lys Thr Ile 
305                 310                 315                 320 

Ser Thr Ser Leu Gly Asn Thr Ser Leu Arg Pro Ser 
                325                 330         


<210>  24

<211>  999

<212>  DNA

<213>  Artificial


<220>

<221>  CDS

<222>  (1)..(999)

<223>  Artificial DNA sequence codon optimised for expression in
       mammalian cells of murine IgE fragment including C2, C3, and C4.


<400>  24

atg gtg aga ccc gtg aac att acc gaa cct aca ctg gag ctg ctc cat       48
Met Val Arg Pro Val Asn Ile Thr Glu Pro Thr Leu Glu Leu Leu His         
1               5                   10                  15              

tcc tct tgt gat cct aac gct ttc cat agc acc att cag ctc tac tgt       96
Ser Ser Cys Asp Pro Asn Ala Phe His Ser Thr Ile Gln Leu Tyr Cys         
            20                  25                  30                  

ttt atc tat ggc cac atc ctg aac gat gtg tct gtc agc tgg ctg atg      144
Phe Ile Tyr Gly His Ile Leu Asn Asp Val Ser Val Ser Trp Leu Met         
        35                  40                  45                      

gat gac cgc gag atc acc gat acc ctc gct cag act gtc ctg atc aaa      192
Asp Asp Arg Glu Ile Thr Asp Thr Leu Ala Gln Thr Val Leu Ile Lys         
    50                  55                  60                          

gaa gag ggc aaa ctc gcc tct act tgt tcc aaa ctg aac atc acc gag      240
Glu Glu Gly Lys Leu Ala Ser Thr Cys Ser Lys Leu Asn Ile Thr Glu         
65                  70                  75                  80          

cag cag tgg atg tcc gaa agc aca ttc acg tgc aag gtg acg agc cag      288
Gln Gln Trp Met Ser Glu Ser Thr Phe Thr Cys Lys Val Thr Ser Gln         
                85                  90                  95              

ggc gtg gac tat ctg gcc cac acc agg cgg tgc ccc gac cac gaa ccc      336
Gly Val Asp Tyr Leu Ala His Thr Arg Arg Cys Pro Asp His Glu Pro         
            100                 105                 110                 

cga ggc gtg att act tac ctg atc cct ccc tcc cct ctg gac ctg tac      384
Arg Gly Val Ile Thr Tyr Leu Ile Pro Pro Ser Pro Leu Asp Leu Tyr         
        115                 120                 125                     

cag aac ggc gct cct aag ctg act tgc ctg gtg gtg gac ctg gag tct      432
Gln Asn Gly Ala Pro Lys Leu Thr Cys Leu Val Val Asp Leu Glu Ser         
    130                 135                 140                         

gag aag aat gtc aat gtc aca tgg aat cag gag aag aag acc tcc gtg      480
Glu Lys Asn Val Asn Val Thr Trp Asn Gln Glu Lys Lys Thr Ser Val         
145                 150                 155                 160         

tct gcc tct cag tgg tac aca aag cac cac aat aac gct acc acc tcc      528
Ser Ala Ser Gln Trp Tyr Thr Lys His His Asn Asn Ala Thr Thr Ser         
                165                 170                 175             

atc aca tct att ctg cca gtg gtc gct aag gac tgg atc gag ggc tat      576
Ile Thr Ser Ile Leu Pro Val Val Ala Lys Asp Trp Ile Glu Gly Tyr         
            180                 185                 190                 

ggc tat cag tgc atc gtc gac cac cca gac ttc ccc aag cct att gtc      624
Gly Tyr Gln Cys Ile Val Asp His Pro Asp Phe Pro Lys Pro Ile Val         
        195                 200                 205                     

aga tct atc aca aag acc cct ggc cag aga agc gct ccc gag gtg tac      672
Arg Ser Ile Thr Lys Thr Pro Gly Gln Arg Ser Ala Pro Glu Val Tyr         
    210                 215                 220                         

gtg ttc ccc cct cca gag gag gag agc gag gat aag aga acc ctg aca      720
Val Phe Pro Pro Pro Glu Glu Glu Ser Glu Asp Lys Arg Thr Leu Thr         
225                 230                 235                 240         

tgt ctg atc cag aat ttt ttt ccc gaa gat att tcc gtg cag tgg ctg      768
Cys Leu Ile Gln Asn Phe Phe Pro Glu Asp Ile Ser Val Gln Trp Leu         
                245                 250                 255             

ggc gat ggc aag ctg att agc aat agc cag cat agc aca aca aca cca      816
Gly Asp Gly Lys Leu Ile Ser Asn Ser Gln His Ser Thr Thr Thr Pro         
            260                 265                 270                 

ctg aaa tcc aac ggc tct aac cag ggc ttt ttc att ttc agc aga ctg      864
Leu Lys Ser Asn Gly Ser Asn Gln Gly Phe Phe Ile Phe Ser Arg Leu         
        275                 280                 285                     

gaa gtg gcc aaa acc ctg tgg acc cag aga aaa cag ttc aca tgc cag      912
Glu Val Ala Lys Thr Leu Trp Thr Gln Arg Lys Gln Phe Thr Cys Gln         
    290                 295                 300                         

gtg atc cat gag gcc ctc cag aaa cca aga aag ctg gaa aag aca atc      960
Val Ile His Glu Ala Leu Gln Lys Pro Arg Lys Leu Glu Lys Thr Ile         
305                 310                 315                 320         

tcc acc tcc ctg ggc aac acc agc ctg aga cct tct taa                  999
Ser Thr Ser Leu Gly Asn Thr Ser Leu Arg Pro Ser                         
                325                 330                                 


<210>  25

<211>  332

<212>  PRT

<213>  mus musculus


<220>

<221>  MISC_FEATURE

<222>  (1)..(332)

<223>  Murine IgE fragment including C2, C3, and C4.


<400>  25

Met Val Arg Pro Val Asn Ile Thr Glu Pro Thr Leu Glu Leu Leu His 
1               5                   10                  15      

Ser Ser Cys Asp Pro Asn Ala Phe His Ser Thr Ile Gln Leu Tyr Cys 
            20                  25                  30          

Phe Ile Tyr Gly His Ile Leu Asn Asp Val Ser Val Ser Trp Leu Met 
        35                  40                  45              

Asp Asp Arg Glu Ile Thr Asp Thr Leu Ala Gln Thr Val Leu Ile Lys 
    50                  55                  60                  

Glu Glu Gly Lys Leu Ala Ser Thr Cys Ser Lys Leu Asn Ile Thr Glu 
65                  70                  75                  80  

Gln Gln Trp Met Ser Glu Ser Thr Phe Thr Cys Lys Val Thr Ser Gln 
                85                  90                  95      

Gly Val Asp Tyr Leu Ala His Thr Arg Arg Cys Pro Asp His Glu Pro 
            100                 105                 110         

Arg Gly Val Ile Thr Tyr Leu Ile Pro Pro Ser Pro Leu Asp Leu Tyr 
        115                 120                 125             

Gln Asn Gly Ala Pro Lys Leu Thr Cys Leu Val Val Asp Leu Glu Ser 
    130                 135                 140                 

Glu Lys Asn Val Asn Val Thr Trp Asn Gln Glu Lys Lys Thr Ser Val 
145                 150                 155                 160 

Ser Ala Ser Gln Trp Tyr Thr Lys His His Asn Asn Ala Thr Thr Ser 
                165                 170                 175     

Ile Thr Ser Ile Leu Pro Val Val Ala Lys Asp Trp Ile Glu Gly Tyr 
            180                 185                 190         

Gly Tyr Gln Cys Ile Val Asp His Pro Asp Phe Pro Lys Pro Ile Val 
        195                 200                 205             

Arg Ser Ile Thr Lys Thr Pro Gly Gln Arg Ser Ala Pro Glu Val Tyr 
    210                 215                 220                 

Val Phe Pro Pro Pro Glu Glu Glu Ser Glu Asp Lys Arg Thr Leu Thr 
225                 230                 235                 240 

Cys Leu Ile Gln Asn Phe Phe Pro Glu Asp Ile Ser Val Gln Trp Leu 
                245                 250                 255     

Gly Asp Gly Lys Leu Ile Ser Asn Ser Gln His Ser Thr Thr Thr Pro 
            260                 265                 270         

Leu Lys Ser Asn Gly Ser Asn Gln Gly Phe Phe Ile Phe Ser Arg Leu 
        275                 280                 285             

Glu Val Ala Lys Thr Leu Trp Thr Gln Arg Lys Gln Phe Thr Cys Gln 
    290                 295                 300                 

Val Ile His Glu Ala Leu Gln Lys Pro Arg Lys Leu Glu Lys Thr Ile 
305                 310                 315                 320 

Ser Thr Ser Leu Gly Asn Thr Ser Leu Arg Pro Ser 
                325                 330         


<210>  26

<211>  999

<212>  DNA

<213>  Artificial



<220>

<221>  CDS

<222>  (1)..(999)

<223>  Artificial DNA sequence codon optimized for expression in 
       E. coli of IgE heavy chain domains C2, C3, and C4

<400>  26

atg gtt cgt ccg gtg aac atc acc gaa cca acg ctg gaa ttg ctg cat       48
Met Val Arg Pro Val Asn Ile Thr Glu Pro Thr Leu Glu Leu Leu His         
1               5                   10                  15              

agc tcc tgc gat ccg aat gct ttt cac agt acc att cag tta tat tgt       96
Ser Ser Cys Asp Pro Asn Ala Phe His Ser Thr Ile Gln Leu Tyr Cys         
            20                  25                  30                  

ttt atc tac ggc cat att ctg aac gat gtc agc gtt tct tgg ctt atg      144
Phe Ile Tyr Gly His Ile Leu Asn Asp Val Ser Val Ser Trp Leu Met         
        35                  40                  45                      

gac gat cgc gag atc act gat acc ctg gcc caa aca gtg ctg att aaa      192
Asp Asp Arg Glu Ile Thr Asp Thr Leu Ala Gln Thr Val Leu Ile Lys         
    50                  55                  60                          

gaa gaa ggt aag ctc gca tca acc tgc tcg aaa ctg aat atc act gag      240
Glu Glu Gly Lys Leu Ala Ser Thr Cys Ser Lys Leu Asn Ile Thr Glu         
65                  70                  75                  80          

cag cag tgg atg agc gaa tcc acc ttc acg tgt aaa gtc act agt caa      288
Gln Gln Trp Met Ser Glu Ser Thr Phe Thr Cys Lys Val Thr Ser Gln         
                85                  90                  95              

ggc gtt gac tat ttg gcg cac acc cgt cgc tgc cct gat cat gag ccg      336
Gly Val Asp Tyr Leu Ala His Thr Arg Arg Cys Pro Asp His Glu Pro         
            100                 105                 110                 

cgt ggg gtc att acc tac ctg atc cca ccg tcg cct tta gat ctg tat      384
Arg Gly Val Ile Thr Tyr Leu Ile Pro Pro Ser Pro Leu Asp Leu Tyr         
        115                 120                 125                     

cag aac gga gcg ccg aag ctc aca tgt ctg gtg gta gac ctt gaa tca      432
Gln Asn Gly Ala Pro Lys Leu Thr Cys Leu Val Val Asp Leu Glu Ser         
    130                 135                 140                         

gag aaa aat gtt aac gtg acc tgg aat cag gaa aag aaa acg tcc gtc      480
Glu Lys Asn Val Asn Val Thr Trp Asn Gln Glu Lys Lys Thr Ser Val         
145                 150                 155                 160         

agc gct agt caa tgg tat acc aag cat cac aac aat gca acg act agc      528
Ser Ala Ser Gln Trp Tyr Thr Lys His His Asn Asn Ala Thr Thr Ser         
                165                 170                 175             

att acc tcc atc ctg cct gtt gtg gcc aaa gat tgg att gaa ggc tac      576
Ile Thr Ser Ile Leu Pro Val Val Ala Lys Asp Trp Ile Glu Gly Tyr         
            180                 185                 190                 

ggt tat cag tgt atc gta gat cat ccg gac ttt cca aaa ccg att gtt      624
Gly Tyr Gln Cys Ile Val Asp His Pro Asp Phe Pro Lys Pro Ile Val         
        195                 200                 205                     

cgc tcg atc acg aag acc cca ggg cag cgt tcc gct cct gaa gtc tac      672
Arg Ser Ile Thr Lys Thr Pro Gly Gln Arg Ser Ala Pro Glu Val Tyr         
    210                 215                 220                         

gtg ttt ccg cct cca gag gaa gaa agt gag gat aaa cgc aca tta acc      720
Val Phe Pro Pro Pro Glu Glu Glu Ser Glu Asp Lys Arg Thr Leu Thr         
225                 230                 235                 240         

tgc ctg att caa aac ttc ttt ccc gaa gat atc agc gtt cag tgg ttg      768
Cys Leu Ile Gln Asn Phe Phe Pro Glu Asp Ile Ser Val Gln Trp Leu         
                245                 250                 255             

ggc gac ggt aaa ctg att tcc aat tca cag cac agc acg act acc ccg      816
Gly Asp Gly Lys Leu Ile Ser Asn Ser Gln His Ser Thr Thr Thr Pro         
            260                 265                 270                 

ctt aag agt aac ggc tcc aat caa ggt ttt ttc atc ttt tcg cgt ctg      864
Leu Lys Ser Asn Gly Ser Asn Gln Gly Phe Phe Ile Phe Ser Arg Leu         
        275                 280                 285                     

gaa gtg gcg aaa acc ctc tgg acg cag cgc aaa cag ttt acc tgt caa      912
Glu Val Ala Lys Thr Leu Trp Thr Gln Arg Lys Gln Phe Thr Cys Gln         
    290                 295                 300                         

gtc att cat gag gca ctg caa aag cct cgt aaa ctg gaa aag aca atc      960
Val Ile His Glu Ala Leu Gln Lys Pro Arg Lys Leu Glu Lys Thr Ile         
305                 310                 315                 320         

agt acc agc tta gga aac acg tcc ctg cgc ccg tcg taa                  999
Ser Thr Ser Leu Gly Asn Thr Ser Leu Arg Pro Ser                         
                325                 330                                 


<210>  27

<211>  332

<212>  PRT

<213>  mus musculus


<220>

<221>  MISC_FEATURE

<222>  (1)..(332)

<223>  Murine IgE fragment including C2, C3, and C4.


<400>  27

Met Val Arg Pro Val Asn Ile Thr Glu Pro Thr Leu Glu Leu Leu His 
1               5                   10                  15      

Ser Ser Cys Asp Pro Asn Ala Phe His Ser Thr Ile Gln Leu Tyr Cys 
            20                  25                  30          

Phe Ile Tyr Gly His Ile Leu Asn Asp Val Ser Val Ser Trp Leu Met 
        35                  40                  45              

Asp Asp Arg Glu Ile Thr Asp Thr Leu Ala Gln Thr Val Leu Ile Lys 
    50                  55                  60                  

Glu Glu Gly Lys Leu Ala Ser Thr Cys Ser Lys Leu Asn Ile Thr Glu 
65                  70                  75                  80  

Gln Gln Trp Met Ser Glu Ser Thr Phe Thr Cys Lys Val Thr Ser Gln 
                85                  90                  95      

Gly Val Asp Tyr Leu Ala His Thr Arg Arg Cys Pro Asp His Glu Pro 
            100                 105                 110         

Arg Gly Val Ile Thr Tyr Leu Ile Pro Pro Ser Pro Leu Asp Leu Tyr 
        115                 120                 125             

Gln Asn Gly Ala Pro Lys Leu Thr Cys Leu Val Val Asp Leu Glu Ser 
    130                 135                 140                 

Glu Lys Asn Val Asn Val Thr Trp Asn Gln Glu Lys Lys Thr Ser Val 
145                 150                 155                 160 

Ser Ala Ser Gln Trp Tyr Thr Lys His His Asn Asn Ala Thr Thr Ser 
                165                 170                 175     

Ile Thr Ser Ile Leu Pro Val Val Ala Lys Asp Trp Ile Glu Gly Tyr 
            180                 185                 190         

Gly Tyr Gln Cys Ile Val Asp His Pro Asp Phe Pro Lys Pro Ile Val 
        195                 200                 205             

Arg Ser Ile Thr Lys Thr Pro Gly Gln Arg Ser Ala Pro Glu Val Tyr 
    210                 215                 220                 

Val Phe Pro Pro Pro Glu Glu Glu Ser Glu Asp Lys Arg Thr Leu Thr 
225                 230                 235                 240 

Cys Leu Ile Gln Asn Phe Phe Pro Glu Asp Ile Ser Val Gln Trp Leu 
                245                 250                 255     

Gly Asp Gly Lys Leu Ile Ser Asn Ser Gln His Ser Thr Thr Thr Pro 
            260                 265                 270         

Leu Lys Ser Asn Gly Ser Asn Gln Gly Phe Phe Ile Phe Ser Arg Leu 
        275                 280                 285             

Glu Val Ala Lys Thr Leu Trp Thr Gln Arg Lys Gln Phe Thr Cys Gln 
    290                 295                 300                 

Val Ile His Glu Ala Leu Gln Lys Pro Arg Lys Leu Glu Lys Thr Ile 
305                 310                 315                 320 

Ser Thr Ser Leu Gly Asn Thr Ser Leu Arg Pro Ser 
                325                 330         


<210>  28

<211>  421

<212>  PRT

<213>  mus musculus


<220>

<221>  MISC_FEATURE

<223>  Murine IgE heavy chain domains C1, C2, C3, and C4.


<400>  28

Ser Ile Arg Asn Pro Gln Leu Tyr Pro Leu Lys Pro Cys Lys Gly Thr 
1               5                   10                  15      

Ala Ser Met Thr Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Asn Pro 
            20                  25                  30          

Val Thr Val Thr Trp Tyr Ser Asp Ser Leu Asn Met Ser Thr Val Asn 
        35                  40                  45              

Phe Pro Ala Leu Gly Ser Glu Leu Lys Val Thr Thr Ser Gln Val Thr 
    50                  55                  60                  

Ser Trp Gly Lys Ser Ala Lys Asn Phe Thr Cys His Val Thr His Pro 
65                  70                  75                  80  

Pro Ser Phe Asn Glu Ser Arg Thr Ile Leu Val Arg Pro Val Asn Ile 
                85                  90                  95      

Thr Glu Pro Thr Leu Glu Leu Leu His Ser Ser Cys Asp Pro Asn Ala 
            100                 105                 110         

Phe His Ser Thr Ile Gln Leu Tyr Cys Phe Ile Tyr Gly His Ile Leu 
        115                 120                 125             

Asn Asp Val Ser Val Ser Trp Leu Met Asp Asp Arg Glu Ile Thr Asp 
    130                 135                 140                 

Thr Leu Ala Gln Thr Val Leu Ile Lys Glu Glu Gly Lys Leu Ala Ser 
145                 150                 155                 160 

Thr Cys Ser Lys Leu Asn Ile Thr Glu Gln Gln Trp Met Ser Glu Ser 
                165                 170                 175     

Thr Phe Thr Cys Lys Val Thr Ser Gln Gly Val Asp Tyr Leu Ala His 
            180                 185                 190         

Thr Arg Arg Cys Pro Asp His Glu Pro Arg Gly Val Ile Thr Tyr Leu 
        195                 200                 205             

Ile Pro Pro Ser Pro Leu Asp Leu Tyr Gln Asn Gly Ala Pro Lys Leu 
    210                 215                 220                 

Thr Cys Leu Val Val Asp Leu Glu Ser Glu Lys Asn Val Asn Val Thr 
225                 230                 235                 240 

Trp Asn Gln Glu Lys Lys Thr Ser Val Ser Ala Ser Gln Trp Tyr Thr 
                245                 250                 255     

Lys His His Asn Asn Ala Thr Thr Ser Ile Thr Ser Ile Leu Pro Val 
            260                 265                 270         

Val Ala Lys Asp Trp Ile Glu Gly Tyr Gly Tyr Gln Cys Ile Val Asp 
        275                 280                 285             

His Pro Asp Phe Pro Lys Pro Ile Val Arg Ser Ile Thr Lys Thr Pro 
    290                 295                 300                 

Gly Gln Arg Ser Ala Pro Glu Val Tyr Val Phe Pro Pro Pro Glu Glu 
305                 310                 315                 320 

Glu Ser Glu Asp Lys Arg Thr Leu Thr Cys Leu Ile Gln Asn Phe Phe 
                325                 330                 335     

Pro Glu Asp Ile Ser Val Gln Trp Leu Gly Asp Gly Lys Leu Ile Ser 
            340                 345                 350         

Asn Ser Gln His Ser Thr Thr Thr Pro Leu Lys Ser Asn Gly Ser Asn 
        355                 360                 365             

Gln Gly Phe Phe Ile Phe Ser Arg Leu Glu Val Ala Lys Thr Leu Trp 
    370                 375                 380                 

Thr Gln Arg Lys Gln Phe Thr Cys Gln Val Ile His Glu Ala Leu Gln 
385                 390                 395                 400 

Lys Pro Arg Lys Leu Glu Lys Thr Ile Ser Thr Ser Leu Gly Asn Thr 
                405                 410                 415     

Ser Leu Arg Pro Ser 
            420     


<210>  29

<211>  33

<212>  DNA

<213>  Artificial


<220>

<221>  CDS

<222>  (1)..(33)

<223>  Artificial DNA sequence encoding epitope in the FG loop of
       murine IgE heavy chain.


<400>  29

gtc gac cac cca gac ttc ccc aag cct att gtc                           33
Val Asp His Pro Asp Phe Pro Lys Pro Ile Val                             
1               5                   10                                  


<210>  30

<211>  11

<212>  PRT

<213>  mus musculus



<220>

<221>  MISC_FEATURE

<222>  (1)..(11)

<223>  Epitope in the FG loop of murine IgE heavy chain.


<400>  30

Val Asp His Pro Asp Phe Pro Lys Pro Ile Val 
1               5                   10      


<210>  31

<211>  36

<212>  DNA

<213>  Artificial


<220>

<221>  CDS

<222>  (1)..(36)

<223>  Artificial DNA sequence encoding epitope in DE loop of murine

       IgE.


<400>  31
aag cac cac aat aac gct acc acc tcc atc aca tct                       36
Lys His His Asn Asn Ala Thr Thr Ser Ile Thr Ser                         
1               5                   10                                  


<210>  32

<211>  12

<212>  PRT

<213>  Artificial


<220>

<221>  MISC-FEATURE

<222>  (1)..(12)

<223>  DE loop of murine IgE.


<400>  32

Lys His His Asn Asn Ala Thr Thr Ser Ile Thr Ser 
1               5                   10          


<210>  33

<211>  27

<212>  DNA

<213>  Artificial


<220>

<221>  CDS

<222>  (1)..(27)

<223>  Artificial DNA sequence encoding epitope in BC loop of murine 
       IgE heavy chain.


<400>  33

ctg gag tct gag aag aat gtc aat gtc                                   27
Leu Glu Ser Glu Lys Asn Val Asn Val                                     
1               5                                                       

<210>  34

<211>  9

<212>  PRT

<213>  Artificial


<220>

<221>  MISC_FEATURE

<222>  (1)..(9)

<223>  Epitope in BC loop of murine IgE heavy chain.


<400>  34

Leu Glu Ser Glu Lys Asn Val Asn Val 
1               5                   


<210>  35

<211>  45

<212>  DNA

<213>  Artificial


<220>

<221>  CDS

<222>  (1)..(45)

<223>  Artificial DNA sequence encoding epitope including linker 
       between the murine IgE heavy chain C2 and C3 domains.


<400>  35

gcc cac acc agg cgg tgc ccc gac cac gaa ccc cga ggc gtg att           45
Ala His Thr Arg Arg Cys Pro Asp His Glu Pro Arg Gly Val Ile             
1               5                   10                  15              


<210>  36

<211>  15

<212>  PRT

<213>  Artificial


<220>

<221>  MISC_FEATURE

<222>  (1)..(15)

<223>  Epitope including linker between the murine IgE heavy chain
       C2 and C3 domains.



<400>  36

Ala His Thr Arg Arg Cys Pro Asp His Glu Pro Arg Gly Val Ile 
1               5                   10                  15  


<210>  37

<211>  27

<212>  DNA

<213>  Artificial



<220>

<221>  CDS

<222>  (1)..(27)

<223>  Artificial DNA sequence encoding epitope including linker 
       between the murine IgE heavy chain C3 and C4 domains.


<400>  37

aca aag acc cct ggc cag aga agc gct                                   27
Thr Lys Thr Pro Gly Gln Arg Ser Ala                                     
1               5                                                       

<210>  38

<211>  9

<212>  PRT

<213>  Artificial


<220>

<221>  MISC_FEATURE

<222>  (1)..(9)

<223>  Epitope including linker between the murine IgE heavy chain
       C3 and C4 domains.


<400>  38

Thr Lys Thr Pro Gly Gln Arg Ser Ala 
1               5                   


