(WO/2001/064024) MULTIPLE GENE EXPRESSION FOR ENGINEERING NOVEL PATHWAYS AND HYPEREXPRESSION OF FOREIGN PROTEINS IN PLANTS
- Biblio. Data
- Description
- Claims
- National Phase
- Notices
- Documents
| Latest bibliographic data on file with the International Bureau
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| IPC: | C07K 14/325 (2006.01), C12N 15/79 (2006.01), C12N 15/82 (2006.01) | ||||||||||||||||||||
| Applicants: | AUBURN UNIVERSITY [US/US]; 309 Samford Hall, Auburn University, AL 36849 (US) (All Except US). UNIVERSITY OF CENTRAL FLORIDA [US/US]; 4000 Central Florida Boulevard, Orlando, FL 32816 (US) (All Except US). DANIELL, Henry [US/US]; (US) (US Only). MOAR, William [US/US]; (US) (US Only). | ||||||||||||||||||||
| Inventors: | DANIELL, Henry; (US). MOAR, William; (US). | ||||||||||||||||||||
| Agent: | WEISER, Gerard, J. et al.; 1600 Market Street, Suite 3600, Philadelphia, PA 19103-7286 (US). | ||||||||||||||||||||
| Priority Data: |
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| Title: | MULTIPLE GENE EXPRESSION FOR ENGINEERING NOVEL PATHWAYS AND HYPEREXPRESSION OF FOREIGN PROTEINS IN PLANTS | ||||||||||||||||||||
| Abstract: | Introducing blocks of foreign genes in a single operon would avoid complications such as position effect and gene silencing inherent in putting one gene at a time into random locations in the nuclear genome. Cloning several genes into a single T-DNA does not avoid the compounded variable expression problem encountered in nuclear transgenic plants. This disclosure shows that a bacterial operon can be expressed in a single integration event as opposed to multiple events requiring several years to accomplish. Expression of multiple genes via a single transformation event opens the possibility of expressing foreign pathways or pharmaceutical proteins involving multiple genes. Expressing the Cry2aA2 operon, including a putative chaperonin to aid in protein folding, in the chloroplast via a single transformation event leads to production of crystalized insecticidal proteins. Expressing the Mer operon via a single transformation event leads to a phytoremediation system. | ||||||||||||||||||||
| Designated States: |
AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZW. African Regional Intellectual Property Org. (ARIPO) (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZW) Eurasian Patent Organization (EAPO) (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM) European Patent Office (EPO) (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, TR) African Intellectual Property Organization (OAPI) (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG). | ||||||||||||||||||||
| Publication Language: | English (EN) |
| Filing Language: | English (EN) |
